Development and validation of UV-spectrophotometric method for the determination of folic acid in bulk and tablet dosage forms
Keywords:Folic acid, Spectrophotometry, Sodium hydroxide, Recovery, Content
The purpose of this study was to create and verify a simple and accurate UV-Spectrophotometric technique for determining Folic acid in bulk and tablet dosage form. The approach was based on the measurement of folic acid solution absorbance at 255nm in 0.01M NaOH. In the concentration range of 10-50 g/ml, the constructed calibration curve was found to be linear (R2 = 0.9996). Limits of detection and quantification were 2.73 µg/ml, and 8.27 µg/ml, respectively. The precision of the developed method was confirmed by the obtained low RSD% values. Recoveries percentages were found 98.46%±1.42, 100.0%±0.20, 98.92%±1.12; n=3 for 50%, 100% and 150% levels, respectively indicating the method accuracy and freedom of interference from excipients. Folic acid content percent in the two analyzed brands were found to be equal 96.59%±0.005; n=3, and 97.28%±0.003; n=3 which complies with the official range (90-110%).
Deconinck E, Crevits S, Baten P, Courselle P, De Beer J. A Validated Ultra High Pressure Liquid Chromatographic Method for Qualification and Quantification of Folic Acid in Pharmaceutical Preparations. J. Pharmac. Biomed. 54, (5), 995-1000 (2011).
Zhao S, Yuan H, Xie C, Xiao D. Determination of Folic Acid by Capillary Electrophoresis with Chemiluminescence Detection. J. Chromatogr. A 1107 (1), 290-293 (2006).
Crane NT, Wilson DB, Cook DA, Lewis CJ, Yetley EA, Rader JI. Evaluating food fortification options: general principles revisited with folic acid. Am J. Publ. Health, 85 (5), 660-666 (1995)
Santos LMP, Pereira MZ. Efeito Da Fortificação Com Ácidofóliconaredução Dos Defeitos Do Tubo Neural. Cad. Saúde Pública, Rio de Janeiro, 23 (1), 17-24 (2007).
Höller U, Brodhag C, Knöbel A, Hofmann P, Spitzer V. Automated determination of selected water-soluble vitamins in tablets using a bench-top robotic system coupled to reversed-phase (RP-18) HPLC with UV detection. Journal of Pharmaceutical and Biomedical Analysis, 31(1), 151-158 (2003).
Iwase H. Determination of folic acid in an elemental diet by high-performance liquid chromatography with UV detection. Journal of Chromatography A, 609(1-2), 399-401 (1992).
Osseyi ES, Wehling RL, Albrecht JA. HPLC determination of stability and distribution of added folic acid and some endogenous folates during breadmaking. Cereal Chemistry, 78 (4), 375 – 378 (2001)
Vahteristo L, Lehikoinen K, Ollilainen V, Varo P. Application of an HPLC assay for the determination of folate derivatives in some vegetables, fruits and berries consumed in Finland. Food Chemistry, 59(4), 589 – 597 (1997)
Ikeda R, Ichiyama K, Tabuchi N, Wada M, Kuroda N, Nakashima K. Determination of Folates by HPLC‐Chemiluminescence using a Ruthenium (II)–Cerium (IV) system, and its application to pharmaceutical preparations and supplements. Luminescence, 29, 824-830 (2014).
Póo-Prieto R, Haytowitz DB, Holden JM, Rogers G, Choumenkovitch SF, Jacques PF, Selhub J. Use of the affinity/HPLC method for quantitative estimation of folic acid in enriched cereal-grain products. J Nutr 136, 3079-83. (2006).
Prasad BB, Tiwari MP, Madhuri R, Sharma PS. Development of a highly sensitive and selective hyphenated technique (molecularly imprinted micro-solid phase extraction fiber–molecularly imprinted polymer fiber sensor) for ultratrace analysis of folic acid. anal. Chim. Acta 662 (1), 14-22 (2010)
Ensafi AA., Karimi-Maleh H. Modified multiwall carbon nanotubes paste electrode as a sensor for simultaneous determination of 6-thioguanine and folic acid using ferrocenedicarboxylic acid as a mediator. J. Electroanal. Chem., 640 (1), 75-83 (2010)
Nie F, He Y, Lu J. An investigation of the chemiluminescence reaction in the sodium hypochlorite-folic acid-emicarbazide hydrochloride system. J. Microchem. 65 (3), 319-323 (2000).
Moura M, Melo ID, Da Costa Lopes FD, Graziella C. Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry. Brazilian Journal of Pharmaceutical Sciences 52, 4 (2016)
Khateeb M, Elias B, Rahal FA. New kinetic spectrophotometric method for determination of folic acid in pharmaceutical formulations. International Letters of Chemistry, Physics and Astronomy, 50, 169-178 (2015).
Patel RK, Patel NM, Shah SK. Development and validation of analytical methods for simultaneous estimation of ferrous ascorbate and folic acid in their combined dosage form. Asian Journal of Pharmaceutical Analysis, 5, 126-132 (2015).
Throat D, Pawar K, Ashwini P. Method development and validation of folic acid by UV-visible spectroscopy. International Journal of Pharmaceutical Sciences and Research, 6, 3088 (2015).
Elimam M, Shantier S, Gadkariem E, Mohamed MA. Development of spectrophotometric methods for the analysis of florfenicol in bulk and dosage forms. Int. J. Pharm. Pharm. Sci., 8(5), 347 – 349 (2016)
Shantier S, Elimam M, Gadkariem E. Difference spectrophotometric methods for the determination of colistin sulphate. Asian J. Pharm. Biochem. Res., 7(4), 1 – 6 (2017)
Shantier S, Garelnabi EA, Gadkariem E. Development of derivative spectrophotometric and HPLC methods for determination of niclosamide. Journal of Harmonized Research in Pharmacy, 4(1), 87-92 (2015).
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