https://japtronline.com/index.php/joapr/issue/feed Journal of Applied Pharmaceutical Research 2025-04-30T00:00:00+00:00 Mr. Aarush Basnet admin@japtronline.com Open Journal Systems <p><em><strong>Journal of Applied Pharmaceutical Research (JOAPR),</strong> <strong>ISSN No. 2348-0335</strong></em> is an official publication of Creative Pharma Assent (CPA). It is an open access, peer reviewed online Journal. JOAPR primarily focuses on publication of manuscript related to multiple disciplines of pharmaceutical sciences (Pharmaceutics, Pharmaceutical Technology, Biopharmaceutics, Cosmetic Technology, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy and Phytochemistry, Herbal drugs/ formulations, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy) and Clinical studies in all areas of human disease and medicine. JOAPR is published bimonthly from August 2023 (earlier quarterly). JOAPR also includes evaluation of pharmaceutical excipients &amp; their practical application to research &amp; industry based efforts. The aim of the scientific journal, JOAPR is to present a wide area for the current researchers to share their noble works and ideas in terms of the research papers, review articles and short communications. JOAPR only publishes original research works with a definite innovation and novelty after thorough plagiarism checking and peer reviewing. The paper must have a suitable and proper scientific background.</p> <p><strong>Brief Information about JOAPR</strong></p> <ul> <li><strong>Journal Title: </strong>Journal of Applied Pharmaceutical Research</li> <li><strong>Journal Abbreviation: </strong>J. Appl. Pharm. Res.</li> <li><strong>Publisher: </strong>Creative Pharma Assent</li> <li><strong>Country: </strong>India</li> <li><strong>Language: </strong>English</li> <li><strong>Publishing Frequency: </strong>Bimonthly (From August 2023)</li> <li><strong>Editor In Chief:</strong> Prof. Amit Roy</li> <li><strong>Editorial Office: </strong>Plot No. 105/42, Opposite electricity sub station, Changorabhata, Raipur (CG) 492001, India</li> <li><strong>Regional Office:</strong> Bishnupath, Rukminigoan, Dispur, Guwahati, Assam, PIN-781022</li> <li><strong>Phone: </strong>+91-8349444385 ; +91-9770019143</li> <li><strong>E-mail: </strong>editor@japtronline.com ; japr.editor@gmail.com</li> <li><strong>Website: </strong>https://www.japtronline.com</li> <li><strong>Publication: </strong>Online only</li> <li><strong>e-ISSN: </strong>2348-0335</li> <li><strong>CODEN: </strong>JAPRIV</li> <li><strong>Year of Start: </strong>2013</li> <li><strong>Review Process</strong><strong>: </strong>Double-blind peer review</li> <li><strong>Indexing: <br />Journal of Applied Pharmaceutical Research is indexed by number of agencies/ organization/ databases like <a href="https://www.scopus.com/sourceid/21101180528" target="_blank" rel="noopener">SCOPUS</a>, Directory of Open Access Journal (DOAJ), Index Copernicus, Crossref, OLCC WorldCat, Garuda, Dimensions, Chemical Abstract Services (CAS), OpenAIRE, Google Scholar, J-Gate, Scilit, International Committee of Medical Journal Editors (ICMJE), Indonesia one search, Indian Citation Index, CNKI, Bielefeld Academic Search Engine (BASE), PKP-Index, Neliti</strong></li> </ul> https://japtronline.com/index.php/joapr/article/view/667 Advancements in smart wearable patch systems for enhanced wound healing 2024-08-24T14:46:28+00:00 Prasanthi Samathoti prasanthiram84@gmail.com Sai Ramya Bodagala sairamya112000@gmail.com <p><strong>Background:</strong> Chronic wounds afflict around 2% of the world's population and cost billions of dollars each year in medical costs. By some estimates, over 13 million people worldwide suffer from chronic wounds yearly. The absence of continuous surveillance in conventional dressings for wounds causes prolongation in the treatment and raises the danger of infection. Timely and practical assessment of wounds is key to reducing infection and healing wounds. This is possible by smart dressings with sensors continuously providing input while monitoring important wound variables involving pH, temperature, and moisture. Considering current and upcoming advancements, this paper examines how intelligent patches could transform healing. <strong>Methodology:</strong> The latest advances in the development, usage of intelligent patches, and their development by different researchers are highlighted in this review. It looks at how sensors are incorporated into these patches and provides an overview of developing intelligent wound dressings by integrating one or more sensors triggered by endogenous and exogenous stimuli. <strong>Results and Discussion:</strong> The fabrication and effectiveness of intelligent dressings have advanced significantly, but there are still issues with sensor precision and resilience, especially regarding the requirement for strict regulations. The discussion also explores the critical need to address legal and technological constraints to enhance the usefulness of such wearable gadgets in medical settings. <strong>Conclusion:</strong> Intelligent patches, a fascinating new development in wound care, enable customized therapy with continuous surveillance. Future studies should address real-world challenges to fully realize their potential to refine wound recovery outcomes in medical care.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Prasanthi Samathoti, Sai Ramya Bodagala https://japtronline.com/index.php/joapr/article/view/676 Nanostructured lipid carriers (NLCs): A comprehensive review of drug delivery advancements 2024-10-27T22:31:31+00:00 Pramesh Panwar prameshpanwar25@gmail.com Saurabh Kumar saurabhkumarbth2@gmail.com Pallavi Chand pallavichand1990@gmail.com Ashish Singh Chauhan ashishchauhan.pharmacy@gmail.com Vikash Jakhmola jakhmola.1979@gmail.com <p><strong>Background: </strong>Nano-structured lipid carriers (NLCs) have emerged as a significant advancement in lipid-based drug delivery systems. Compared to Solid Lipid Nanoparticles (SLNs), NLCs offer improved drug loading capacity, stability, and controlled release profiles. <strong>Objective:</strong> This review article explores the structural and functional benefits of NLCs, their enhanced bioavailability, and their potential in targeted therapeutic agent delivery. <strong>Methodology:</strong> We investigated the unique combination of solid and liquid lipids in NLCs, which creates an internal structure for improved drug encapsulation and sustained release. Various methods were analyzed for their contribution to NLC efficacy, including high-pressure homogenization and solvent emulsification. <strong>Results and Discussion:</strong> NLCs demonstrated encapsulation efficiency ranging from 85–95%, with particle sizes between 100–300 nm. Drug release was sustained over 24 hours, affirming their effectiveness in controlled drug delivery. Applications of NLCs were highlighted across diverse delivery modes, including topical, pulmonary, oral, parenteral, and ocular. Challenges such as scaling production, overcoming biological barriers, and ensuring regulatory compliance were also addressed. <strong>Conclusion:</strong> NLCs present a promising future in pharmaceutical sciences, with the potential to improve therapeutic outcomes in complex diseases like cancer and neurodegenerative disorders. Continued research into NLC formulations is critical for advancing patient outcomes and addressing global health challenges.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Pramesh Panwar, Saurabh Kumar, Pallavi Chand, Ashish Singh Chauhan, Vikash Jakhmola https://japtronline.com/index.php/joapr/article/view/953 A comprehensive review of peptic ulcer disease: epidemiology, experimental models, and mechanistic insights 2025-03-02T16:45:09+00:00 Laliteswar Pratap Singh lalitpharma@gmail.com Sandip Chatterjee sandipkarna1994@gmail.com Sanjeeb Kumar Kar sanjeeb.bit@gmail.com <p><strong>Background: </strong>Peptic ulcer disease (PUD) is one of the most common gastrointestinal disorders, resulting from an imbalance between aggressive and protective factors, leading to mucosal erosion. Various factors influence its pathogenesis, including Helicobacter pylori infection, NSAID use, and oxidative stress. <strong>Objective: </strong>We review PUD epidemiology, varying experimental models, and mechanistic insights into PUD and promising therapeutics. <strong>Methodology: </strong>We systematically reviewed previous literature on PUD, including epidemiological trends, commonly used in vivo, and molecular mechanisms. <strong>Results and discussion: </strong>The global prevalence of peptic ulcer disease (PUD) follows an epidemiological pattern influenced by geography, lifestyle, and genetic factors. Experimental models using ethanol, NSAIDs, or <em>Helicobacter pylori</em> induction provide valuable insights into disease progression and pathophysiology. <strong>Emerging trends</strong>: Recent research in peptic ulcer disease focuses on molecular mechanisms, gut microbiome interactions, personalized therapies, and novel pharmacological agents. Molecular studies explore genetic and epigenetic factors influencing ulcer formation, while microbiome research examines the role of gut bacteria beyond <em>H. pylori</em>. Personalized treatment approaches use genetic profiling and biomarkers to enhance efficacy and reduce toxicity. Additionally, emerging pharmacological agents aim to improve acid suppression, promote mucosal healing, and develop more effective <em>H. pylori</em> eradication strategies. <strong>Conclusion: </strong>A deeper understanding of PUD pathophysiology through epidemiological studies and experimental models can aid in developing novel, targeted therapies. Future research should focus on alternative treatments, including phytochemicals and probiotics, to enhance ulcer prevention and management.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Laliteswar Pratap Singh, Sandip Chatterjee, Sanjeeb Kumar Kar https://japtronline.com/index.php/joapr/article/view/825 Advancements in formulations and technologies for colon-targeted drug delivery 2025-03-07T21:17:07+00:00 Ritik Singh Rana ritiksinghrana@gmail.com Yogita Ale yogitaale7@gmail.com Pankaj Pant pankaj.pant@dituniversity.edu.in Neha Kukreti nehakukreti11@gmail.com Vikash Jakhmola Jakhmola.1979@gmail.com <p><strong>Background:</strong> Colonic administration of drugs may enhance drug absorption, reduce adverse reactions, and facilitate delivery to specific therapeutic targets. <strong>Objective:</strong> Delivering pharmaceuticals to the colon poses challenges that require innovative formulation strategies. <strong>Methodology</strong>: Various formulation approaches have been explored for colon-targeted drug delivery systems. These approaches target the colon using formulation components that interact with GI physiology parameters such as pH, colonic flora, and enzymes. <strong>Result and Discussion:</strong> The article discussed the various research studies conducted for colon targeting involving novel strategies such as pH-dependent, enzyme-dependent, Ligand-Receptor-based, new technologies, Phloral, and magnetically derived approaches. It also explored the translational technologies, such as in vivo, in vitro, and in silico, which expedite the transition from fundamental research to clinical application and enhance therapeutic outcomes. <strong>Conclusion:</strong> In conclusion, the most relevant preclinical studies, encompassing in vitro, in vivo, and in silico research, are delineated to facilitate the strategic advancement of novel colon-targeted therapeutics.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Ritik Singh Rana, Yogita Ale, Pankaj Pant, Neha Kukreti, Vikash Jakhmola https://japtronline.com/index.php/joapr/article/view/1006 Chitosan-coated CMC and carbopol hydrogel beads for controlled release of metformin in diabetes management 2025-03-03T05:04:33+00:00 Sachin Gupta guptasachin.250798@gmail.com Swati Dubey swatidubey211@gmail.com Sanjeev Kumar Patel sanjeevpy@gmail.com Anshu Priyanka Lakra anshu.tem@aiimsbhopal.edu.in Sunita Minz sunita.minz@igntu.ac.in <p><strong>Background:</strong> Current research aims to fabricate carboxymethyl cellulose sodium (CMC-Na) and carbopol hydrogel beads. Gleichzeitig, beads were coated with chitosan to enhance the controlled release of the drug Metformin HCl (MET), which serves as a model drug for diabetes mellitus (DM). <strong>Methodology:</strong> The MET beads were synthesized through the ionotropic gelation process. The foundation of ionotropic gelation is a polyelectrolyte’s capacity to cross-link to create hydrogels when counterions are present. The negatively charged carboxylate groups (-COO⁻) on CMC-Na form electrostatic interactions with the positively charged aluminium ions (Al³⁺) from AlCl<sub>3</sub>. The quality-by-design approach was employed to optimize process factors in preparing hydrogel beads. A comprehensive evaluation of the beads covered various aspects such as particle size, scanning electron microscopy, percentage yield, Fourier transform infrared spectroscopy, X-ray diffraction, entrapment efficiency (EE), and <em>in vitro </em>drug release. <strong>Results and Discussion:</strong> The beads were spherical, with an average particle diameter of 153.6 to 231.5 μm. The entrapment efficiency percentage range is 94.4% and 97.83% for MET-loaded and chitosan-coated MET-loaded beads, respectively. Therefore, <em>in-vitro</em> drug release of the optimized MET-loaded beads is 55.5 %, and chitosan-coated MET-loaded beads are approximately 48.8% achieved in 10 hours. <strong>Conclusion: </strong>Chitosan-coated CMC-Na and carbopol hydrogel beads showed good MET encapsulation and sustained release, improving structural integrity and drug release. The ionotropic gelation process created stable, homogeneous beads, making this delivery method viable for oral sustained-release MET formulations.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Sachin Gupta, Swati Dubey, Sanjeev Kumar Patel, Anshu Priyanka Lakra, Sunita Minz https://japtronline.com/index.php/joapr/article/view/688 Exploring the therapeutic potential of Eleusine indica plant: promising antioxidant and analgesic activity with lack of antimicrobial and thrombolytic activity 2024-09-03T21:19:42+00:00 Farhana Israt Jahan jifarhana@daffodilvarsity.edu.bd Asif Hossain Anik asifhossain2994@gmail.com Sumaiya Akter sumaiyaakhain@gmail.com <p><strong>Background:</strong> <em>Eleusine indica</em> is used as a traditional medicine in Bangladesh. It is significantly valued for its wound-healing properties and is often used as an anthelmintic and pain reliever in rural areas. <strong>Objective:</strong> This study examines the pharmacological effects of <em>Eleusine indica</em> methanolic extracts, highlighting antioxidant, antimicrobial, thrombolytic, and analgesic properties in animal models. Also, contribute to future research directions. <strong>Methods:</strong> The antioxidant properties were determined using the DPPH free radical scavenging assay, with ascorbic acid as the positive control, and the inhibition percentage was calculated. Antimicrobial activity was determined using the disc diffusion method. The thrombolytic Potential was determined through in vitro clot lysis assays. However, the % of clot lysis was used to gauge the thrombolytic effects. For assessing analgesic activity, Swiss albino mice were utilized; analgesic efficacy was evaluated by calculating the percentage inhibition against abdominal writhing. <strong>Results:</strong> The free radical scavenging assay demonstrated with an IC<sub>50</sub> of 43.67 μg/ml, comparable to ascorbic acid's IC<sub>50</sub> of 36.22 μg/ml. However, the methanolic extract showed no antimicrobial activity. In thrombolytic assays, the extract induced 26.79% clot lysis. With a 55.57% reduction in writhing, the extract group (500 mg/kg) exhibited significant analgesic activity, approaching the standard group's 73% inhibition. <strong>Conclusion: </strong>The Outcome of the study shows <em>Eleusine indica</em> has potential antioxidant and analgesic activity, with a lack of antimicrobial and thrombolytic properties. The results support the application of traditional medicine. highlighting the importance of antioxidant and analgesic activity, in future investigations aimed at isolating and identifying the specific compounds driving its pharmacological activities.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Farhana Israt Jahan, Asif Hossain Anik, Sumaiya Akter https://japtronline.com/index.php/joapr/article/view/943 Design, development, and optimization of mucoadhesive buccal films of ganaxolone for enhanced bioavailability 2025-02-06T06:02:59+00:00 Onkar Pawar pawaronkar2001@gmail.com Rahul Godge rahulgodge@gmail.com Ganesh Shinde shinde.ganesh904@gmail.com Kailas Barde bkailas495@gmail.com Akshay Vikhe vikhe.akshay2001@gmail.com <p><strong>Background:</strong> CDD disorder affects children mainly during their first three months of life. The buccal route offers advantages over oral administration for ganaxolone by avoiding first-pass metabolism and providing direct systemic absorption. This study aimed to formulate and characterise mucoadhesive buccal films of ganaxolone to increase its bioavailability. <strong>Methods:</strong> Mucoadhesive buccal films were prepared using a solvent casting technique employing HPMC K4M and Moringa gum as polymers. The formulation was optimized using a 3<sup>2</sup>-factorial design, where polymer concentrations were varied systematically to achieve optimal film properties. Nine batches (OF1-OF9) were formulated and evaluated for various physicochemical parameters, mucoadhesive strength, percentage drug content, goat buccal mucosa permeation study, and stability analysis. <strong>Results:</strong> Based on the findings, the OF8 batch containing optimal polymer ratio (250mg HPMC K4M and 60mg Moringa gum) emerged as the superior formulation with 94.45±0.34% drug content, 15.37±0.58 N/mm² tensile strength, and 7.8±0.57 N mucoadhesive strength. Permeation studies consequently confirmed 96.37% of the drug at 8 hours with a 13.63 µg /cm² /h permeation rate. There was no evidence of drug-excipient interaction in FTIR and DSC analysis. The formulation was set to be stable for 6 months at accelerated conditions (40±2°C, 75±5% RH) with an average tensile strength above 15 N/mm² and an average <em>ex-vivo</em> drug permeation of 93%. <strong>Conclusion:</strong> This optimized buccal film formulation demonstrates promising potential for clinical application in CDD treatment by offering enhanced bioavailability, controlled release, and patient-friendly administration, which is particularly beneficial for pediatric patients.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Onkar Pawar, Rahul Godge, Ganesh Shinde, Kailas Barde, Akshay Vikhe https://japtronline.com/index.php/joapr/article/view/907 Formulation and evaluation of diclofenac emulgel using natural permeation enhancers 2025-01-24T06:43:11+00:00 Chandra Sekhar Naik D chandu.desavath@gmail.com Shaik Noor Mahammed shaiknoor14325@gmail.com Mudigedu Samreen mudigedusamreen13@gmail.com S. Vamsi vamsinani213@gmail.com P. Hemalatha Pagadalahema0@gmail.com <p><strong>Background</strong>: This study aimed to formulate a stable diclofenac emulgel and determine the penetration rate using different natural penetration enhancers. <strong>Methodology</strong>: Carbopol (934) and Hydroxypropyl methyl cellulose (HPMC) were utilized as gelling agents due to their favorable viscosity characteristics, which render them widely used for regulating the flow properties of topically administered dosage forms. In the research, diclofenac served as the active ingredient, while Carbopol (934) &amp; HPMC (0.5%) acted as gelling forms to form a proper gel base. Emulsion contains Tween-20 (0.05%), PEG (0.6%), liquid paraffin (0.75%), span-20 (0.1%), along with natural penetration enhancers (0.3%) initially prepared gel base &amp; the emulsion with natural penetration enhancers combine conjointly to shape an appropriate diclofenac emulgel. <strong>Results and discussion: </strong>According to the study, the improved batch exhibits a 95.08% release in 48 hours and remains stable for about three. The optimized batch exhibits 46.6% suppression in the microbiological assay, whereas the marketed treatment only demonstrates 32.3% inhibition. However, the skin irritation test results have no erythema or edema. The rabbits' skin showed no signs of discomfort. According to stability experiments, the synthesized emulgel's antifungal activity, rheological analysis, in vitro drug release, and physical appearance did not alter after three months of storage. <strong>Conclusion</strong>: Overall, it was recommended that, in contrast to cream, the emulgel formulation come after the drug release for controlled, long-term drug delivery.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Chandra Sekhar Naik D, Shaik Noor Mahammed, Mudigedu Samreen, S. Vamsi, P. Hemalatha https://japtronline.com/index.php/joapr/article/view/985 Antioxidant and hepatoprotective activity of Nigella sativa alcoholic extract in a CCl4-induced rat 2025-03-19T06:16:14+00:00 A.S. Devadhe amardevadhepatil9977@gmail.com Santosh Dighe santosh.dighe@pravara.in S.S. Yadav yadashubham29@gmail.com S. B Bhawar sbbhawar@gmail.com R D Ghogare rajashri.ghogare@pravara.in <p><strong>Background: </strong>This study investigated the antioxidant, hepatoprotective, and sedative modulatory effects of <em>Nigella sativa</em> alcoholic extract (NSAE) in CCl<sub>4</sub>-induced hepatotoxicity in rats. <strong>Methods</strong>: Male Wistar rats were divided into six groups (n=6): normal control, CCl<sub>4</sub> control, silymarin (50 mg/kg), and NSAE (100, 200, and 400 mg/kg). Hepatoprotective effects were evaluated through biochemical parameters, oxidative stress markers, and histopathological examination. <strong>Results</strong>: NSAE treatment (400 mg/kg) significantly restored liver function markers, including SGOT (20.95 ± 0.52 IU/L, p = 0.033) and SGPT (28.61 ± 0.67 IU/L, p &lt; 0.001), compared to CCl<sub>4</sub> control. Total protein and albumin levels were normalized to 5.68 ± 0.54 mg/dL and 3.84 ± 0.48 mg/dL, respectively. Antioxidant parameters showed marked improvement with NSAE (400 mg/kg), increasing GSH (0.26 ± 0.029 µmol/mg) and CAT (30.19 ± 2.69 µg/mL) while reducing MDA (0.048 ± 0.008 µg/mL). Histopathological examination revealed significant protection against CCl<sub>4</sub>-induced hepatic and gastric tissue damage, particularly at the 400 mg/kg. <strong>Conclusion</strong>: NSAE exhibited marked hepatoprotective activity comparable to silymarin, predominantly through antioxidant mechanisms and the maintenance of hepatic tissue integrity, indicating its potential as a natural therapeutic agent for managing liver diseases. Because of its hepatoprotective and antioxidant properties, NSAE may be explored in clinical settings as a natural supplement to traditional liver disease therapies or as a prophylactic for people at risk of liver disorders.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 A.S. Devadhe, Santosh Dighe, S.S. Yadav, S. B Bhawar, R D Ghogare https://japtronline.com/index.php/joapr/article/view/1008 Exploring 1,3,4-oxadiazole derivatives for hepatocellular carcinoma: synthesis, and bioactivity evaluation 2025-04-02T20:34:40+00:00 Mohini Patidar mohinipatidar321@gmail.com Raghvendra Dubey raghuji22@gmail.com Nitin Deshmukh nitdeshmukh90@gmail.com <p><strong>Background: </strong>Cancer is a leading cause of death globally, with existing treatments often limited by resistance and toxicity. This necessitates the development of new, more effective anticancer therapies.</p> <p><strong>Methodology: </strong>This study used <em>In-silico</em> modeling with tools like Pre-ADMET and Molinspiration to evaluate the physicochemical, pharmacokinetic, and pharmacodynamic properties of substituted 1,3,4-Oxadiazole derivatives. <strong>Results and discussion: </strong>Computational studies of 1,3,4-Oxadiazole analogues showed promising drug-like properties and bioavailability. To test the inhibitory efficacy against the protein target tyrosine kinase (PDB: 1M17), 30 designed derivative compounds underwent molecular docking experiments. 10 synthesized derivatives were structurally confirmed through Mass<strong>, </strong>NMR<strong>,</strong> and IR spectrometry, ensuring their purity and identity. Molecular docking and in vitro tests identified compound S23 as a potent tyrosine kinase inhibitor, with significant anti-proliferative activity (GI50: 0.25665634) and enzyme inhibition (IC50: 1.87), highlighting its potential as a therapeutic agent. <strong>Conclusion:</strong> According to our findings, the substituted derivative might offer superior potential for developing anticancer medicine. </p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Mohini Patidar, Raghvendra Dubey , Nitin Deshmukh https://japtronline.com/index.php/joapr/article/view/867 Quality by design driven RP-HPLC method optimization for analysis of levothyroxine and liothyronine in bulk and tablet dosage form 2025-03-07T19:46:49+00:00 Ramdas Darade rdarade4@gmail.com Sanjay Pekamwar sspekamwar@gmail.com <p><strong>Background: </strong>Levothyroxine and Liothyronine are widely used in thyroid hormone replacement therapies. Simultaneously quantifying Levothyroxine and Liothyronine is important for managing thyroid hormone deficiency. <strong>Aim: </strong>This study aims to develop and validate an accurate and robust RP-HPLC method for simultaneously quantifying Levothyroxine and Liothyronine by utilizing Quality by Design (QbD). <strong>Methodology:</strong> Reversed phase chromatography was performed using a High Performance Liquid Chromatographic System (Agilent Technologies Ltd, 1100 series) equipped with a UV detector. The column used was Agilent C 18 (100 mm x 4.6 mm; 5µm) HPLC Column. The chromatographic separation was carried out using a mobile phase composed of Methanol and Formic acid (0.1%) (50:50 %v/v) with a flow rate of 1.2 ml/min, and a UV detector recorded the response at 254 nm. Design expert was used as software to evaluate experimental design studies (Stat-Ease Inc., Minneapolis, USA, Version 13.0). <strong>Result and Discussion: </strong>The RP-HPLC method was established to quantify Levothyroxine and Liothyronine simultaneously. The established method was linear, and correlation coefficients (R<sup>2</sup>) were 0.9993 and 0.9994 for Levothyroxine and Liothyronine, respectively. Retention times of Levothyroxine and Liothyronine were 2.587 minutes and 3.035 minutes. Results of accuracy, precision studies, LOD, and LOQ were found within acceptable limits. <strong>Conclusion: </strong>A robust RP-HPLC method was developed for the simultaneous quantification of levothyroxine and liothyronine by utilizing a QbD. The QbD technique provided a systematic methodology for identifying and optimizing the critical parameters influencing the method's performance.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Ramdas Darade, Sanjay Pekamwar https://japtronline.com/index.php/joapr/article/view/951 Identification of novel potential benzimidazole derivatives by pharmacophore generation, 3D-QSAR, virtual screening, molecular docking and ADME/ TOX analysis against breast cancer as targeted estrogen alpha receptor 2025-03-30T20:05:22+00:00 Aastha Sharma aasthasharma2397@gmail.com Nitish Banga nitishbanga123@gmail.com Rakesh Kumar Marwaha rkmarwaha.mdu@gmail.com Balasubramanian Narasimhan naru2000us@gmail.com <p><strong>Background: </strong>The estrogen alpha receptor (ERα) is critical in breast carcinogenesis. Although selective estrogen receptor modulators like tamoxifen are clinically used, their adverse effects highlight the need for safer alternatives. The study uses computational methods to identify potential ERα inhibitors within a benzimidazole scaffold. <strong>Methodology: </strong>This study employed computational approaches, including pharmacophore generation, 3D-QSAR, virtual screening, molecular docking, and in silico ADME/Tox analysis. The best pharmacophore model (DDRRR_1) identified two hydrogen donors and three aromatic rings as critical features. Moreover, a rigorous external validation was used on decoy databases with optimized metrics (ROC, BEDROC, AUROC). A subsequent atom-based 3D-QSAR model with a high correlation coefficient (R² = 0.9), cross-validated coefficient (Q² = 0.8), and Fisher ratio (F = 80.1) was developed. Benzimidazole scaffolds from PubChem were screened, followed by docking against ERα (PDB ID: 3ERT) and ADMET profiling. <strong>Results and Discussion: </strong>The pharmacophore model validated the importance of the identified features. The 3D-QSAR model effectively screened benzimidazole scaffolds, with five component PLS factors, supporting the pharmacophore findings. This model effectively screened benzimidazole scaffolds obtained from the PubChem database, followed by molecular docking against the targeted protein ERα (PDB ID: 3ERT) and identified five promising compounds. ADME/Tox profiling revealed PubChem ID 3074802 (2-[2-(1H-indol-3-yl) ethyl]1H-benzimidazole) has favourable pharmacokinetics and a low toxicity profile. <strong>Conclusion: </strong>These findings indicate that PubChem ID 3074802 is a promising candidate for further therapeutic drug development in breast cancer treatment. It demonstrates the highest binding affinity (-9.842 kcal/mol) compared to the standard drug Tamoxifen (-5.357 kcal/mol) and exhibits a favorable ADME/Tox profile.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Aastha Sharma, Nitish Banga, Rakesh Kumar Marwaha, Balasubramanian Narasimhan https://japtronline.com/index.php/joapr/article/view/997 Development and evaluation of an amorphous solid dispersion-based probucol immediate-release tablet 2025-03-11T01:26:27+00:00 Vaibhav Adhikari vbhvadhikari@gmail.com Mansi Butola mansibutola1995@gmail.com Vikash Jakhmola jakhmola.1979@gmail.com Abhijeet Ojha ojhaabhijeet24@gmail.com Arvind Negi arvindnegi85@gmail.com <p><strong>Background:</strong> In its crystalline form, probucol has an extremely low bioavailability and is a poor water-soluble drug. The main aim of this study was to enhance the solubility and dissolution rate of probucol by using a solvent evaporation method to develop a solid dispersion that contains polyvinyl pyrrolidone K30 (PVP-K30) and polyethylene glycol 6000 (PEG 6000). <strong>Methodology:</strong> The solvent evaporation method is considered superior to other techniques for preparing solid dispersions due to its ability to achieve uniform drug distribution at the molecular level. This method ensures homogeneity by dissolving the drug and carrier in a common solvent, reducing the risk of drug recrystallization and enhancing solubility and bioavailability. <strong>Result: </strong>The drug-to-carrier ratio is the determining factor for dissolution enhancement. The FTIR spectra do not suggest any chemical interaction between PVP-K30 or PEG 6000. The immediate release profiles of both formulations were favourable, with F3 releasing approximately 95.31% of the drug and F6 releasing around 86.77% within 2 hours. This indicates a rapid drug dissolution, which is beneficial for achieving a fast onset of action and enhancing bioavailability. <strong>Conclusion:</strong> The solid dispersion formulations F3 &amp; F6 successfully transformed crystalline probucol to an amorphous state, enhancing solubility &amp; dissolving rates appropriate for immediate-release tablets.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Vaibhav Adhikari, Mansi Butola, Vikash Jakhmola, Abhijeet Ojha, Arvind Negi https://japtronline.com/index.php/joapr/article/view/1093 Development and characterisation of lyophilised ethambutol-loaded polymeric nanoparticles 2025-04-18T05:40:38+00:00 Eknath Kole koleeknath.111@gmail.com Yogesh Sonar yshsonar@gmail.com Rahul J. Sarode rahulmicro55@gmail.com Atul Chaudhari atulchaudhari7420@gmail.com Jitendra Naik jitunaik@gmail.com <p><strong>Background</strong>: Tuberculosis (TB) remains a universal health crisis, requiring innovative drug delivery systems to overcome challenges like prolonged treatment duration and patient non-adherence. This study was designed to develop Ethambutol (ETH)-loaded poly-ε-caprolactone (PCL) nanoparticles (NPs) as a sustained-release pulmonary delivery platform for TB therapy. <strong>Methodology</strong>: ETH-PCL NPs were fabricated using the nanoprecipitation technique with Lutrol® F68 as a stabiliser. The formulation was optimised for physicochemical properties (particle size, polydispersity index (PDI), zeta potential), encapsulation efficiency (EE), and morphology (SEM). <em>In vitro</em> drug release and 3-month colloidal stability were evaluated. <strong>Results and Discussion</strong>: The optimised NPs exhibited a rod-shaped morphology with smooth surfaces, an average size of 426.3 ± 13.03 nm, PDI &lt; 0.467, zeta potential of -18.8 ± 0.520 mV, and EE of 76.57±3.86%. Sustained ETH release (86.62% over 24 h) and robust colloidal stability (negligible changes in size, PDI, and zeta potential over 3 months) were achieved. The formulation's biodegradable PCL core and scalable design align with the need for cost-effective, patient-centric therapies. <strong>Conclusion</strong>: ETH-PCL NPs represent a promising nanocarrier platform for TB, combining sustained drug release, high encapsulation efficiency, and long-term stability. While in vitro results are encouraging, future studies must validate in vivo efficacy and pulmonary delivery potential. This work underscores the viability of nanotechnology in addressing TB treatment challenges, particularly in improving adherence and targeting mycobacteria-laden macrophages.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Eknath Kole, Yogesh Sonar, Rahul J. Sarode, Atul Chaudhari, Jitendra Naik https://japtronline.com/index.php/joapr/article/view/1037 Formulation and optimization of upadacitinib-loaded transdermal patches for rheumatoid arthritis with zero-order release kinetics 2025-03-29T06:13:13+00:00 Shubham Talole Shubhamtalole2003@gmail.com Rahul Godge rahulgodge@gmail.com Nikita Tambe nikitatambe26@gmail.com Nikita Mhase htcnikitamhase@gmail.com <p><strong>Background: </strong>To develop and optimize Upadacitinib-loaded transdermal patches for rheumatoid arthritis treatment with improved patient compliance and sustained drug delivery. <strong>Methodology:</strong> Upadacitinib transdermal patches were formulated using a 3² factorial design approach with PVP K30 and HPMC K4M as key polymeric components. The patches were characterized for physicochemical, mechanical, and ex vivo permeation properties. <strong>Results and Discussion:</strong> The optimized formulation (SF8) exhibited excellent physicochemical characteristics, including high drug content (99.05 ± 0.83%), optimal mechanical properties with tensile strength of 0.912 kg/mm² and adhesion strength of 3.94 N. The ex vivo permeation reached 86.35% at 12h, with the flux of 102.91 μg/cm²/h following zero-order kinetics (R² = 0.9777). The experimental values closely matched predicted values with less than 2% error. Accelerated stability studies confirmed minimal changes in critical parameters over six months. <strong>Conclusion:</strong> The optimized Upadacitinib transdermal patch provides sustained drug delivery with zero-order release kinetics and excellent stability. This transdermal delivery system offers a promising alternative to oral therapy with potential advantages of improved patient compliance, reduced dosing frequency, and avoidance of first-pass metabolism for rheumatoid arthritis management</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Shubham Talole, Rahul Godge, Nikita Tambe, Nikita Mhase https://japtronline.com/index.php/joapr/article/view/1031 A mechanism-driven strategy for in-silico prediction, molecular docking, synthesis, and biological assessment of substituted 1,3,4-oxadiazole derivatives as novel antidiabetic agents 2025-04-11T09:16:50+00:00 Mohini Patidar mohinipatidar321@gmail.com Raghvendra Dubey raghuji22@gmail.com Sunita Minz sunita.minz@igntu.ac.in Madhulika Pradhan madhulika.pradhan1@gmail.com Nitin Deshmukh nitdeshmukh90@gmail.com <p><strong>Background</strong>: Diabetes mellitus is a long-standing and debilitating metabolic condition that imposes a substantial global health burden, leading to severe and widespread complications. <strong>Objectives</strong>: This study aims to predict physicochemical properties of 1,3,4-oxadiazole derivatives using in-silico methods and molecular docking simulations to explore their potential as α-glucosidase inhibitors for diabetes management. Furthermore, this study aims to experimentally synthesize and characterize these derivatives to validate their inhibitory activity. <strong>Methods</strong>: In silico drug-likeness, pharmacokinetic, and toxicity profiling of substituted oxadiazole derivatives were performed using the Molinspiration and PreADMET web tools. Molecular docking simulations were conducted with the target protein alpha-glucosidase (PDB ID: 3WY1) to assess its anti-diabetic potential. This study suggests that oxadiazole has the potential to be a novel anti-diabetic agent. <strong>Results</strong>: Compound 3a1 formed 5 significant hydrogen bonds with Gly228, Thr226, Leu227, Tyr235, Glu271 with docking scores of -156.118 and re-rank scores of -91.600 comparable to the standard drug Miglitol, which formed 6 hydrogen bonds Val380, Asp401, Lys398, Gly399, Glu377, Asp379 but had lower docking and re-rank scores (-69.4415 and -95.887). Based on docking results, five oxadiazole derivatives were synthesized via Mannich base cyclization, yielding 62.2 – 79.9%. They showed moderate to excellent anti-diabetic activity, with compounds 3a1 and 3a3 demonstrating no toxicity or mortality at 40 mg/kg oral dose. <strong>Conclusion</strong>: Our study highlights that the oxadiazole pharmacophore is a key structural motif for the development of potential anti-diabetic compounds</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 Mohini Patidar, Sunita Minz, Madhulika Pradhan, Raghvendra Dubey, Nitin Deshmukh https://japtronline.com/index.php/joapr/article/view/977 Development of a stability-indicating UPLC method for quantification of mirvetuximab soravtansine-gynx in pharmaceutical formulations using quality by design (QbD) principles 2025-03-11T19:10:16+00:00 K. V. Umamaheswara Rao kundurthisankar5@gmail.com Neetu Shorgar nshorgar@gmail.com <p><strong>Background: </strong>This study intended to introduce a robust ultra-performance liquid chromatography (UPLC) method for quantifying Mirvetuximab soravtansine-gynx (MSG) in pharmaceutical dosage forms. A systematic approach incorporating the Design of Experiments (DoE) was employed to optimize reliable, sensitive, and efficient chromatographic conditions. <strong>Methodology:</strong> The finalized method utilized a Waters ACQUITY BEH Phenyl (50 mm) Column with a mobile phase comprising acetonitrile and 0.1% aqueous formic acid in 30:70 (v/v) at 0.2 mL/min and 271 nm and PDA wavelength. <strong>Results and discussion:</strong> The method validation demonstrates excellent linearity (R² = 0.991, p &lt; 0.05) over 17.50–105 µg/mL. The Intraday and interday precision (%RSD) values were 0.568 and 0.544, respectively, confirming method reproducibility. Accuracy was validated through recovery studies, which produced results within the range of 100.1–100.5%, whereas the robustness test highlights the method's resilience to minor variations. This method detects MSG at a very low concentration of 0.42 µg/mL, confirming method sensitivity. The forced degradation studies were conducted under various stress conditions. The result suggests that moderate degradation of 10.3%, 14.5%, and 9.5 % was noticed in acidic, peroxide, and reduction (9.5%) conditions. <strong>Conclusion:</strong> The purity analyses confirm the absence of significant impurities in the stress degradation chromatogram, highlighting the stability of MSG and the reliability of the proposed method. In conclusion, the proposed method was rapid, sensitive, precise, robust, and stable for quantifying MSG in pharmaceutical formulations.</p> 2025-04-30T00:00:00+00:00 Copyright (c) 2025 K. V. Umamaheswara Rao, Neetu Shorgar