HR-LCMS based metabolites profiling, pharmacognostic study, and antimycotic activity of leaves of Ruellia asperula

Arshu Patel; J R Kudnar; R S Jadhav; B Ghuge

doi:10.69857/joapr.v13i1.743

Authors

Arshu Patel professor
J R Kudnar Pravara Rural College of Pharmacy, Pravaranagar, Maharashtra, India 413736
R S Jadhav Pravara Rural College of Pharmacy, Pravaranagar, Maharashtra, India 413736
B Ghuge Pravara Rural College of Pharmacy, Pravaranagar, Maharashtra, India 413736

DOI:

https://doi.org/10.69857/joapr.v13i1.743

Keywords:

Ruellia asperula, HR-LCMS, metabolite profiling, antimycotic activity, phytochemicals, pharmacognosy

Abstract

Background: Fungal infections pose a global health challenge, exacerbated by rising drug resistance and immunocompromised populations. Ruellia asperula, a traditional medicinal plant, has garnered attention for its bioactive compounds, including flavonoids and alkaloids. This study aims to profile its metabolites using HR-LCMS and evaluate its antimycotic potential, contributing to the discovery of natural therapeutic agents. Methodology: Leaves and bark of Ruellia asperula were collected, authenticated, and processed for analysis. Physicochemical standards like moisture content, ash values, and extractive yields were determined. Preliminary phytochemical screening identified bioactive compounds. Ethanolic extracts were prepared via Soxhlet extraction, fractionated through column chromatography, and analyzed using HR-LCMS. In vitro, antimycotic assays were conducted against Alternaria and Macrophomia. Results: Physicochemical analysis revealed a total ash content of 2.87% w/w, water-soluble ash of 25.78% w/w, and alcohol-soluble extractive value of 9.53% w/w, indicating substantial secondary metabolites. Phytochemical screening identified alkaloids, flavonoids, and saponins. HR-LCMS analysis detected 18 compounds in the ethanolic fraction and 13 in the chloroform fraction. In vitro assays demonstrated significant inhibition of Alternaria and Macrophomia, with activity comparable to Itraconazole. Discussion: Ruellia asperula leaves demonstrated high phytochemical quality and are rich in flavonoids and alkaloids. HR-LCMS profiling identified bioactive metabolites, while in vitro tests confirmed significant antifungal activity. These findings underscore the plant's potential as a natural antimycotic agent. Conclusion: This study highlights the phytochemical richness of Ruellia asperula, confirmed by HR-LCMS and pharmacognostic analyses. Its potent antimycotic activity, comparable to Itraconazole, positions it as a promising candidate for natural fungal therapies

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References

Limper A, Adenis A, Le T, Harrison T. Fungal infections in HIV/AIDS. Lancet Infect Dis., 17, e334-e343 (2019) https://doi.org/10.1016/S1473-3099(17)30303-1.

Lilienfeld T, Wagener J, Einsele H, Cornely O, Kurzai O. Invasive Fungal Infection. Dtsch Ärztebl Int., 116, 271-278 (2019) https://doi.org/10.3238/arztebl.2019.0271.

Friendman D, Schwartz I. Emerging Fungal Infections: New Patients, New Patterns, and New Pathogens. Journal of Fungi., 5, (2019) https://doi.org/10.3390/jof5030067.

Suleyman G, Alangaden G. Nosocomial Fungal Infections: Epidemiology, Infection Control, and Prevention. Infect Dis Clin., 30, 1023-1052 (2016) https://doi.org/10.1016/j.idc.2016.07.008.

Enoch D, Yang H, Aliyu S, Micallef C. The Changing Epidemiology of Invasive Fungal Infections. Hum. Fungal Pathog. Identif. Methods Protoc., 1508, 17-65 (2017) https://doi.org/10.1007/978-1-4939-6515-1_2.

Manzitto-Tripp E, Daniel T. Phylogeny and revised classification of New World Ruellia. TAXON., 72, 1034–1056 (2023) https://doi.org/10.1002/tax.13001.

Sianturi G, Trisnawati E, Koketsu M, Suryanti V. Chemical constituents and antioxidant activity of Britton’s wild petunia (Ruellia brittoniana) flower. Biodiversitas J Biol Divers., 24, (2023) https://doi.org/10.13057/biodiv/d240703.

Do V, Do L, Mai V. Investigation of in vitro biological activity from extracts of Ruellia tuberosa. Pak J Biol Sci., 27, 224–233 (2024) https://doi.org/10.3923/pjbs.2024.224.233.

Freitas A, Rodrigues P. Seed Morphology of Ruellieae Species (Acanthaceae) in Brazil and Its Taxonomic Implications. Syst Bot, 44, 631–51 (2019) https://doi.org/10.1600/036364419X15620113920662.

Samy M, Sugimoto S, Matsunami K, Otsuka H, Kamel M.S. Chemical constituents and biological activities of genus Ruellia. Int J Pharmacognosy., 2, 270–279 (2015) http://dx.doi.org/10.13040/IJPSR.0975-8232.IJP.2(6).270-79.

Salunke M, Wakure B, Wakte P. High-resolution liquid chromatography and mass spectrometry (HR-LCMS) assisted phytochemical profiling and an assessment of anticancer activities of Gracilaria foliifera and Turbinaria conoides using in vitro and molecular docking analysis. J Biomol Struct Dyn., 41, 6476–91 (2023) https://doi.org/10.1080/07391102.2022.2108495.

Reddy M, Adnan M, Alreshidi M, Saeed M, Patel M. Evaluation of Anticancer, Antibacterial and Antioxidant Properties of a Medicinally Treasured Fern Tectaria coadunata with its Phytoconstituents Analysis by HR-LCMS. Anti-Cancer Agents Med Chem., 20, 1845–56 (2020) https://doi.org/10.2174/1871520620666200318101938.

Koomson D, Kwakye B, Darkwah W, Odum B, Asante M, Aidoo G. Phytochemical Constituents, Total Saponins, Alkaloids, Flavonoids and Vitamin C Contents of Ethanol Extracts of five Solanum torvum Fruits. Pharmacogn J., 10, 946–50 (2018) https://doi.org/10.5530/pj.2018.5.160.

Alvarez-Rivera G, Ballesteros-Vivas D, Parada-Alfonso F, Ibañez E, Cifuentes A. Recent applications of high-resolution mass spectrometry for the characterization of plant natural products. TrAC Trends Anal Chem., 112, 87–101 (2019) https://doi.org/10.1016/j.trac.2019.01.002.

Dubey S, Maity S, Singh M, Saraf S.A, Saha S. Phytochemistry, Pharmacology and Toxicology of Spilanthes acmella: A Review. Advances in Pharmacological Sciences., 2013, 1-9 (2023) https://doi.org/10.1155/2013/423750.

Warsinah, Baroroh H.N. Pharmacognostic Profile of Ageratum conyzoides L Plant and Simplicia. Pharmacognosy Journal., 12,1072–1076 (2020) https://doi.org/10.5530/pj.2020.12.151.

Bokov D, Nizamova L, Morokhina S, Marakhova A, Bobkova N, Sergunova E. Pharmacognostic studies of Origanum L. species medicinal plant raw materials. Res J Pharm Technol., 13, 4365-4372 (2020) https://doi.org/10.5958/0974-360X.2020.00772.6.

Ray A, Rahaman C. Pharmacognostic, Phytochemical and Antioxidant Studies of Gardenia latifolia Aiton: An Ethnomedicinal Tree Plant. Int J Pharmacogn Phytochem Res., 10 (2018).

Nafees M, Barkatullah, Ullah S, Ikram N. Phytochemical and pharmacognostic studies of Buddleja asiatica leaves. Microsc Res Tech., 85, 510–20 (2022) https://doi.org/10.1002/jemt.23924.

Kaur S, Arora S. Phytopharmacological evaluation of potential medicinal plants. Pharmacogn Rev., 3,152–59 (2009) https://doi.org/10.4103/0973-7847.65332.

Pande J, Chaudhari S, Nilam R. Limonium stocksii: A pharmacognostic and phytochemical evaluation of a halophyte from Gujarat. Int J Curr Microbiol Appl Sci., 7, 1234–1240 (2018) https://doi.org/10.20546/ijcmas.2018.705.145.

García-Bores A, Álvarez-Santos N, López-Villafranco M, Jácquez-Ríos M, Aguilar-Rodríguez S, Grego-Valencia D. Verbesina crocata: A pharmacognostic study for the treatment of wound healing. Saudi J Biol Sci., 27, 3113–24 (2020) https://doi.org/10.1016/j.sjbs.2020.08.038.

Tang G, Lin X, Lai X, Gong X, Ji S. Pharmacognostic Studies of Psychotria rubra(Lour.)Poir. Pharmacogn J., 10, 249–55 (2018) https://doi.org/10.5530/pj.2018.2.44.

Kanakiya A, Padalia H, Pande J, Chanda S. Physicochemical, Phytochemical and Pharmacognostic study of Limonium stocksii, a halophyte from Gujarat. J Phytopharm, 7, 312–318 (2018) https://doi.org/10.31254/phyto.2018.7314.

Noumi E, Snoussi M, Anouar E, Alreshidi M, Veettil V, Elkahoui S. HR-LCMS-Based Metabolite Profiling, Antioxidant, and Anticancer Properties of Teucrium polium L. Methanolic Extract: Computational and In Vitro Study. Antioxidants., 9, 1089 (2020) https://doi.org/10.3390/antiox9111089.

Singh P, Singh J, Medhi T, Kumar A. Phytochemical Screening, Quantification, FT-IR Analysis, and In Silico Characterization of Potential Bio-active Compounds Identified in HR-LC/MS Analysis of the Polyherbal Formulation from Northeast India. ACS Omega., 7, 33067–78 (2022) https://doi.org/10.1021/acsomega.2c03117.

Marulasiddaswamy K, Nuthan B, Sunilkumar C, Bajpe S, Kumara K, Sekhar S. HR-LC-MS based profiling of phytochemicals from methanol extracts of leaves and bark of Myristica dactyloides Gaertn. J Appl Biol Biotechnol., 9, 124–35 (2021) https://doi.org/10.7324/JABB.2021.9517.

Muzzalupo I, Badolati G, Chiappetta A, Picci N, Muzzalupo R. In vitro Antifungal Activity of Olive (Olea europaea) Leaf Extracts Loaded in Chitosan Nanoparticles. Front Bioeng Biotechnol., 8, (2020) https://doi.org/10.3389/fbioe.2020.00151.

Trong Le N, Viet Ho D, Quoc Doan T, Tuan Le A, Raal A, Usai D. In Vitro Antimicrobial Activity of Essential Oil Extracted from Leaves of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le in Vietnam. Plants., 9, 453 (2020) https://doi.org/10.3390/plants9040453.

Ho H, Le T, Dao T, Le T, Dinh T, Nguyen D. Development of Itraconazole-Loaded Polymeric Nanoparticle Dermal Gel for Enhanced Antifungal Efficacy. J Nanomater., 2020, (2020) https://doi.org/10.1155/2020/8894541.

Hanafy S, Abd El-Shafea Y, Saleh W, Fathy H. Chemical profiling, in vitro antimicrobial and antioxidant activities of pomegranate, orange and banana peel-extracts against pathogenic microorganisms. J Genet Eng Biotechnol, 19, (2021) https://doi.org/10.1186/s43141-021-00151-0.