In vitro antibacterial and synergistic activity of pyrazolyl sulphonamide derivatives against Staphylococcus haemolyticus

Ewura Seidu Yahaya; Jesse Azebiik  Anak; Nana Ama  Amissah; Collins Obuah; Phyllis Elsie Owusu  Agyei; Roland Osei  Saahene; Michael  Ainooson; Anthony  Ablordey; Martins  Ekor

doi:10.69857/joapr.v12i5.658

Authors

Ewura Seidu Yahaya Department of Pharmacology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Ghana
Jesse Azebiik Anak Department of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Ghana
Nana Ama Amissah Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
Collins Obuah Department of Chemistry, University of Ghana, Legon, Ghana
Phyllis Elsie Owusu Agyei Department of Pharmacology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Ghana https://orcid.org/0009-0002-4010-0594
Roland Osei Saahene Department of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Ghana
Michael Ainooson Department of Chemistry, University of Ghana, Legon, Ghana
Anthony Ablordey Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
Martins Ekor Department of Pharmacology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Ghana

DOI:

https://doi.org/10.69857/joapr.v12i5.658

Keywords:

Antibacterial activity, Antimicrobial resistance, Sulphonamide derivatives, Synergistic activity, Staphylococcus haemolyticus

Abstract

Background: Antimicrobial resistance has rendered several anti-infective agents ineffective, necessitating the need to intensify efforts to identify and develop novel drugs against microbial infection. Structural modification of existing antibiotics continues to be one of the areas of intense research focus in recent times. Objective: This study assessed the antibacterial and synergistic activity of fifteen pyrazolyl sulphonamide derivatives in Staphylococcus haemolyticus. Methods: Antibacterial activity was determined using the broth microdilution method. The ability of test compounds to interact with tetracycline was assessed using the checkerboard synergy testing method and the Loewe synergy and antagonism model. Results: Seven compounds (46.6%) were significantly active against the bacteria (MIC 1 µg/mL – 16 µg/mL), and four were confirmed to form synergistic combinations with tetracycline in checkerboard and Loewe analysis. Conclusion: Observations from this study has demonstrated the antibacterial activity and the synergistic potential of the novel pyrazolyl sulphonamides with tetracycline, highlighting the possible role of modified sulphonamides as a rich resource for antimicrobial development.

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