Preparation and evaluation of antibacterial mupirocin cream emulsion using cocamidopropyl betaine emulsifier

Avinash B Gangurde; Suraj Pagar

doi:10.69857/joapr.v12i6.633

Authors

Avinash B Gangurde Department of Pharmaceutics, K.B.H.S.S. Trusts Institute of Pharmacy, Malegaon Camp, Malegaon, Nashik, Maharashtra 423105
Suraj Pagar Department of Pharmaceutics, K.B.H.S.S. Trusts Institute of Pharmacy, Malegaon Camp, Malegaon, Nashik, Maharashtra 423105

DOI:

https://doi.org/10.69857/joapr.v12i6.633

Keywords:

Antibacterial, cream , emulsion, mupirocin, Staphylococcus aureus

Abstract

Background: This study aimed to develop and evaluate an antibacterial cream emulsion containing mupirocin using Cocamidopropyl betaine (CAPB) as an emulsifier. Mupirocin, a topical antibiotic effective against Staphylococcus aureus (including methicillin-resistant strains), was formulated into a cream to enhance its topical delivery. Materials and Methods: Mupirocin cream emulsion formulations were developed with varying concentrations of CAPB, PEG-400, and glycerol monostearate. The cream formulations were mainly evaluated for in vitro diffusion tests, antibacterial activity tests, and stability studies. Result and Discussion: CAPB produced a stable cream emulsion formulation (F7) at 30% concentration and 2% PEG-400. The formulation (F7) exhibited sustained drug release over 3.5 hours in the diffusion test. The formulation F7 showed a higher zone of inhibition, 32.16±2.2 mm, than the marketed mupirocin cream, 29.56±1.35 mm, for the Staphylococcus aureus strain. The prepared cream formulation F7 was found stable over 90 days at different temperature conditions (8±2°C, 25±2°C and 40±2°C). Conclusion: The study concludes that CAPB effectively enhances mupirocin cream solubility and antibacterial properties, making it a promising option for treating bacterial skin infections.

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References

Lee AS, de Lencastre H, Garau J,Kluytmans J, Malhotra-Kumar S, Peschel A, Harbarth S. Methicillin-resistant Staphylococcus aureus. Nat Rev Dis Primers, 4, 18033 (2018) https://doi.org/10.1038/nrdp.2018.33

Roth GA, Abate D, Abate KH, Abay SM, Abbafati C, Abbasi N, et al. Global, regional, and national age-sex-specific mortality for 282 causes of death in 195 countries and territories, 1980–2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet, 392, 10159 (2018) https://doi.org/10.1016/S0140-6736(18)32203-7

Miró EM, Sánchez NP. Cutaneous manifestations of infectious diseases. Atlas of Dermatology in Internal Medicine. (Sánchez NP ed.) Springer, New York, pp. 77-119 (2011) https://doi.org/10.1007/978-1-4614-0688-4_7

Dadashi M, Hajikhani B, Darban-Sarokhalil D, Van Belkum A, Goudarzi M. Mupirocin resistance in Staphylococcus aureus: a systematic review and meta-analysis. J Glob Antimicrob Resist, 20, 238-47 (2020) https://doi.org/10.1016/j.jgar.2019.07.032

Hemmingsen LM, Škalko-Basnet N, Jøraholmen MW. The expanded role of chitosan in localized antimicrobial therapy. Mar Drugs, 19, 697 (2021) https://doi.org/10.3390/md19120697

Li M, Zhu X, Cao Z, Du L, Wei J, Zhang C. Fire needle combined with topical mupirocin for the treatment of simple skin abscesses in pediatric patients: a case series. Infect Drug Resist, 16, 3683-92 (2023) https://doi.org/10.2147/IDR.S416917

Rajagopal M, Walker S. Envelope structures of gram-positive bacteria. Current Topics in Microbiology and Immunology. Springer International Publishing, pp. 1-44 (2015) https://doi.org/10.1007/82_2015_5021

Khoshnood S, Heidary M, Asadi A, Soleimani S, Motahar M, Savari M, Saki M, Abdi MA. A review on the mechanism of action, resistance, synergism, and clinical implications of mupirocin against Staphylococcus aureus. Biomed Pharmacother, 109, 1809-18 (2019) https://doi.org/10.1016/j.biopha.2018.10.131

Gangwar A, Kumar P, Singh R, Kush P. Recent advances in mupirocin delivery strategies for the treatment of bacterial skin and soft tissue infection. Future Pharmacol, 1, 80-103 (2021) https://doi.org/10.3390/futurepharmacol1010007

Jacob SE, Amini S. Cocamidopropyl betaine. Dermatitis, 19, 157–60 (2008) https://doi.org/10.2310/6620.2008.06043

Waglewska E, Bazylińska U. Biodegradable amphoteric surfactants in titration-ultrasound formulation of oil-in-water nanoemulsions: rational design, development, and kinetic stability. Int J Mol Sci, 22, 11776 (2021) https://doi.org/10.3390/ijms222111776

Gisby J, Bryant J. Efficacy of a new cream formulation of mupirocin: comparison with oral and topical agents in experimental skin infections. Antimicrob Agents Chemother, 44, 255-60 (2000) https://doi.org/10.1128/AAC.44.2.255-260.2000

Alhasso B, Ghori MU, Conway BR. Development of nanoemulsions for topical application of mupirocin. Pharmaceutics, 15, 378 (2023) https://doi.org/10.3390/pharmaceutics15020378

Zubairi MB, Abd AH, Al-lami MS. Formulation and characterization of mupirocin nano micelles in insulin-based gel for dermatological application. J Pharm Bioallied Sci, 15, S1178-81 (2023) https://doi.org/10.4103/jpbs.jpbs_172_23

Suxam S, Bharti N, Sharma P, Kumari N. Microwave-generated bionanocomposites for solubility and dissolution enhancement of poorly water-soluble drug. Asian J Pharm Res, 12, 192-8 (2022) https://doi.org/10.52711/2231-5691.2022.00031

Bhat R, Dogra A, Chib S, Kumar M, Khan IA, Nandi U, Saran S. Development of mupirocin-impregnated bacterial cellulosic transdermal patches for the management of skin infection. ACS Omega, 9, 5496-5508, (2024) https://doi.org/10.1021/acsomega.3c07174

Chen MX, Alexander KS, Baki G. Formulation and evaluation of antibacterial creams and gels containing metal ions for topical application. J Pharm (Cairo), 5754349 (2016) https://doi.org/10.1155/2016/5754349

Purnamawati S, Indrastuti N, Danarti R, Saefudin T. The role of moisturizers in addressing various kinds of dermatitis: a review. Clin Med Res, 15, 75-87 (2017) https://doi.org/10.3121/cmr.2017.1363

Ermawati DE, Surya AP, Setyawati R, Niswah SU. The effect of glycerin and polyethylene glycol 400 as humectants on stability and antibacterial activity of nanosilver biosynthetic peel-off mask. J Appl Pharm Sci, 12, 80-89 (2022) https://doi.org/10.7324/JAPS.2022.120409

Torfs E, Brackman G. A perspective on the safety of parabens as preservatives in wound care products. Int Wound J, 18, 221-32 (2021) https://doi.org/10.1111/iwj.13521

Kim D, Seok JK, Kim M, Choi S, Hong J, Yoon YA, Chung H, Bae ON, Kwack SJ, Kim KB, Lee JY. Safety assessment of cocamidopropyl betaine, a cosmetic ingredient. Toxicol Res, 40, 361–375 (2024) https://doi.org/10.1007/s43188-024-00243-2

Adejokun DA, Dodou K. A Novel method for the evaluation of the long-term stability of cream formulations containing natural oils. Cosmetics, 7, 86 (2020) https://doi.org/10.3390/cosmetics7040086

Elena OB, Maria NA, Michael SZ, Natalia BD, Alexander I , Ivan IK. Dermatologic gels spreadability measuring methods: a comparative study. Int J App Pharm, 14, 164-68 (2022) https://doi.org/10.22159/ijap.2022v14i1.41267

Tan PL, Rajagopal M, Chinnappan S, Selvaraja M, Leong MY, Tan LF, Yap VL. Formulation and physicochemical evaluation of green cosmeceutical herbal face cream containing standardized mangosteen peel extract. Cosmetics. 9, 46 (2022) https://doi.org/10.3390/cosmetics9030046

Ong MWS, Simion FA, Maibach HI. Methods for testing irritation potential. Textbook of Hand Eczema. (Alikhan A., Lachapelle JM., Maibach H. eds.) Springer, Berlin, pp. 233-46 (2014) https://doi.org/10.1007/978-3-642-39546-8_22

Ng SF, Rouse J, Sanderson D, Eccleston GA. A comparative study of transmembrane diffusion and permeation of ibuprofen across synthetic membranes using Franz diffusion cells. Pharmaceutics, 2, 209-23 (2010) https://doi.org/10.3390/pharmaceutics2020209

Tansathien K, Opanasopit P, Patrojanasophon P, Kumpugdee-Vollrath M. Comparison of fish skins as alternative biological skin models for skin permeability study. Science Engineering and Health Studies, 17, 23050016 (2023) https://doi.org/10.69598/sehs.17.23050016

Borse VA, Gangude AB, Deore AB. Formulation and evaluation of antibacterial topical gel of doxycycline hyclate, neem oil, and tea tree oil. Indian J Pharm Educ Res, 54, 206-12 (2020) https://doi.org/10.5530/ijper.54.1.24

Almeida LQ, Do Nascimento LD, da Costa FAM, Da Costa KS, Andrade EHA. In vitro antibacterial activity and in silico analysis of the bioactivity of major compounds obtained from the essential oil of Virola surinamensis Warb. J Food Qual, 2022, 5275805 (2022) https://doi.org/10.1155/2022/5275805

Smaoui S, Hlima HB, Chobba IB, Kadri A. Development and stability studies of sunscreen cream formulations containing three photo-protective filters. Arab J Chem, 10, S1216-22 (2017) https://doi.org/10.1016/j.arabjc.2013.02.020

Manna K, Panda AK. Physicochemical studies on the interfacial and micellization behaviour of CTAB in aqueous polyethylene glycol media. J Surfactants Detergents, 14, 563-76 (2011) https://doi.org/10.1007/s11743-011-1261-8