Formulation and evaluation of ointment containing hydroalcoholic extract derived from the bark of Moringa oleifera for wound healing activity in rat model

Authors

  • Himanshu Sahu Columbia Institute of Pharmacy, Raipur, 493111, Chhattisgarh, India
  • Trilochan Satapathy Columbia Institute of Pharmacy, Raipur, 493111, Chhattisgarh, India
  • Shashikant Chandrakar Columbia Institute of Pharmacy, Raipur, 493111, Chhattisgarh, India
  • Puahpa Prasad Gupta Chhatrapati Shivaji Institute of Pharmacy, Durg, 491001, Chhattisgarh, India
  • Poonam Sahu Columbia Institute of Pharmacy, Raipur, 493111, Chhattisgarh, India
  • Akhilesh Sahu Chhatrapati Shivaji Institute of Pharmacy, Durg, 491001, Chhattisgarh, India

DOI:

https://doi.org/10.69857/joapr.v12i4.556

Keywords:

Wound healing, Physico-chemical, Phytochemical, Antioxidant, Histopathology

Abstract

Background: This study aimed to assess the effectiveness of a hydroalcoholic extract derived from the bark of Moringa oleifera in facilitating the healing process of second-degree burns wounds. Moreover, a comprehensive assessment was carried out on standardized M. oleifera bark to ascertain its physiochemical characteristics, botanical compound layout, and antioxidant activity, all of which play a crucial role in its capacity to facilitate the healing process of burns. Methods: For 14 days, the efficacy of ointments containing a hydroalcoholic extract of M. oleifera bark at concentrations of 5% and 10% was evaluated for treating second-degree burns in rats. Additionally, histological analysis was conducted on skin tissue samples. Results: The M. oleifera bark extract exhibited TPC (52.56 mg/gm of dried extract) and TFC (84.33 mg/gm of dried extract) value along with antioxidant activity (IC50 value of 0.98 µg/ml) for radical scavenging, in the presence of several phytochemicals. The most favorable outcomes were achieved using a 10% ointment composition, demonstrating a wound closure and tissue repair rate of 83.04 ± 0.89%, along with a noteworthy decrease in tissue oxidative stress indicators. Histological investigations have verified the wound-healing properties of M. oleifera bark extract. Conclusion: Due to its significant antioxidant properties and its capacity to create a moist environment for wounds, M. oleifera has the potential to serve as a natural treatment for burns. Additional clinical trials are recommended to validate the efficacy of M. oleifera bark extract as a therapeutic agent for wound healing.

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Published

2024-08-31

How to Cite

Sahu, H., Satapathy, T., Chandrakar, S., Gupta, P. P., Sahu, P., & Sahu, A. (2024). Formulation and evaluation of ointment containing hydroalcoholic extract derived from the bark of Moringa oleifera for wound healing activity in rat model. Journal of Applied Pharmaceutical Research, 12(4), 44-53. https://doi.org/10.69857/joapr.v12i4.556

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Articles