Formulation and evaluation of ointment containing hydroalcoholic extract derived from the bark of Moringa oleifera for wound healing activity in rat model

Himanshu Sahu; Trilochan Satapathy; Shashikant Chandrakar; Puahpa Prasad Gupta; Poonam Sahu; Akhilesh Sahu

doi:10.69857/joapr.v12i4.556

Authors

Himanshu Sahu Columbia Institute of Pharmacy, Raipur, 493111, Chhattisgarh, India
Trilochan Satapathy Columbia Institute of Pharmacy, Raipur, 493111, Chhattisgarh, India
Shashikant Chandrakar Columbia Institute of Pharmacy, Raipur, 493111, Chhattisgarh, India
Puahpa Prasad Gupta Chhatrapati Shivaji Institute of Pharmacy, Durg, 491001, Chhattisgarh, India
Poonam Sahu Columbia Institute of Pharmacy, Raipur, 493111, Chhattisgarh, India
Akhilesh Sahu Chhatrapati Shivaji Institute of Pharmacy, Durg, 491001, Chhattisgarh, India

DOI:

https://doi.org/10.69857/joapr.v12i4.556

Keywords:

Wound healing, Physico-chemical, Phytochemical, Antioxidant, Histopathology

Abstract

Background: This study aimed to assess the effectiveness of a hydroalcoholic extract derived from the bark of Moringa oleifera in facilitating the healing process of second-degree burns wounds. Moreover, a comprehensive assessment was carried out on standardized M. oleifera bark to ascertain its physiochemical characteristics, botanical compound layout, and antioxidant activity, all of which play a crucial role in its capacity to facilitate the healing process of burns. Methods: For 14 days, the efficacy of ointments containing a hydroalcoholic extract of M. oleifera bark at concentrations of 5% and 10% was evaluated for treating second-degree burns in rats. Additionally, histological analysis was conducted on skin tissue samples. Results: The M. oleifera bark extract exhibited TPC (52.56 mg/gm of dried extract) and TFC (84.33 mg/gm of dried extract) value along with antioxidant activity (IC₅₀ value of 0.98 µg/ml) for radical scavenging, in the presence of several phytochemicals. The most favorable outcomes were achieved using a 10% ointment composition, demonstrating a wound closure and tissue repair rate of 83.04 ± 0.89%, along with a noteworthy decrease in tissue oxidative stress indicators. Histological investigations have verified the wound-healing properties of M. oleifera bark extract. Conclusion: Due to its significant antioxidant properties and its capacity to create a moist environment for wounds, M. oleifera has the potential to serve as a natural treatment for burns. Additional clinical trials are recommended to validate the efficacy of M. oleifera bark extract as a therapeutic agent for wound healing.

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