Evaluation of the hepatoprotective and nephroprotective properties of bael fruit extract against carbon tetrachloride-induced toxicity in rats

Deepak Garg; Amit  Sharma; Pragi; Varun Kumar

doi:10.69857/joapr.v12i3.524

Authors

Deepak Garg Department of Pharmacy, Faculty of Medical, Paramedical and Allied Health Sciences, Jagannath University, Jaipur, Rajasthan 303901
Amit Sharma Faculty of Medical, Paramedical and Allied Health Sciences, Jagannath University, Jaipur, Rajasthan 303901
Pragi Department of Pharmacy, Jagannath University Bahadurgarh, Haryana 124507
Varun Kumar Department of Pharmacy, Jagannath University Bahadurgarh, Haryana 124507

DOI:

https://doi.org/10.69857/joapr.v12i3.524

Keywords:

Bael Fruit, Biochemical parameters, Hepatoprotective, Nephroprotective, Histopathology

Abstract

Background: Bael is well-known for its antibacterial properties. Aqueous fruit extracts have also been shown to have hepatoprotective properties; the nephroprotective and hepatoprotective properties of Ethanolic extracts have not yet been tested. Objective: To evaluate the hepatoprotective and nephroprotective activities of Bael against CCl₄-induced toxicity in rats. Methods: Two dosages of Bael's Ethanolic extract (100 and 200 mg/kg/day) were compared with 100 mg/kg of silymarin. Histopathologic alterations of the liver and kidney, as well as biochemical blood parameters such as bilirubin, urea, uric acid, total protein and creatinine, alkaline phosphatase (ALP), alanine aminotransferase (ALT), were examined and assessed. Results: Bael was more successful in lowering high levels of urea, creatinine, ALT, AST, and ALP when he used a 200 mg/kg/day methanol extract. According to the histopathologic assessment, Bael lessened the CCl4-induced hepatic and renal necrosis. The more significant dose resulted in reductions in AST, ALT, GGT, ALP, and bilirubin of 45,25, 52,36, and 16%, respectively. Ethanolic extract 200 mg/kg/day also shows a reduction in elevated levels of Creatinine, Urea, Uric Acid, and Total Protein by 57%, 52%, 34%, and 9%, respectively. Conclusion: There were established hepatoprotective and nephroprotective effects of the Bael fruit methanol extract, with 200 mg/kg/day being the most beneficial dose. This provides scientific proof that medicinal herbs like Bael can be used to treat renal and liver diseases.

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References

Eugenio-Pérez D, Montes de Oca-Solano HA, Pedraza-Chaverri J. Role of food derived antioxidant agents against acetaminophen induced hepatotoxicity. Pharmaceutical Biology, 54, 2340-2352 (2016)

Daudon M, Frochot V, Bazin D, Jungers P. Drug-induced kidney stones and crystalline nephropathy: pathophysiology prevention and treatment. Drugs, 78,163–201(2018).

Bhar, K., Mondal S, Suresh P. An Eye-Catching Review of Aegle marmelos L. (Golden Apple). Pharmacognosy Journal, 11: 207-224 (2019).

Suksomboon N, Poolsup N, Boonkaew S, Suthisisang CC. Meta-analysis of the effect of herbal supplement on glycemic control in type 2 diabetes. J Ethnopharmacol, 3, 1328-1333 (2011)

Mohamed N.Z, Abd Alla H.I, Aly H.F, Ibrahim N.A., Hassan S.A. CCl4-induced hepato-nephrotoxicity: protective effect of nutraceuticals on inflammatory factors and antioxidative status in rat. J. Appl. Pharm. Sci, 4, 87–100 (2014)

Bansal Y, Bansal G. Analytical methods for standardization of Aegle marmelos. A Review. J. Pharm. Educ. Res, 2, 37–44 (2011)

Singh, Vijay K., Thomas M. Seed. The Efficacy and Safety of Amifostine for the Acute Radiation Syndrome. Expert Opinion on Drug Safety , 11, 1077–90 (2019)

Cheema AK, Li Y, Girgis M, Jayatilake M, Fatanmi oo, Wise SY, Seed TM, Singh VK. Alterations in Tissue Metabolite Profiles with Amifostine-Prophylaxed Mice Exposed to Gamma Radiation. Metabolites, 10, 2-14 (2020)

Warwas B, Cremers F, Gerull K, Leichtle A, Bruchhage KL, Hakim SG, Schild SE, Rades D. Risk Factors for Xerostomia Following Radiotherapy of Head and Neck Cancers. Anticancer Research, 42, 2657-2663 (2022)

Anders MW. Metabolism of drugs by the kidney. Kidney Int, 18, 636-47 (1980)

Karimi, A, Majlesi, M, Rafieian-Kopaei, M. Herbal versus synthetic drugs; beliefs and facts. Journal of Nephropharmacology, 4, 27-30 (2015).

Hahn G, Lehmann HD, Kurten M, Uebel H, Vogel G. On the pharmacology and toxicology of silymarin, an antihepatotoxic active principle from Silybum marianum (L.) Gaertn. Arzneimittelforschung, 18, 698–704 (1968)

Ferenci P, Dragosics B, Dittrich H, Frank H, Benda L, Lochs H, Meryn S, Base W, Schneider B. Randomized controlled trial of silymarin treatment in patients with cirrhosis of the liver. J Hepatol. 9, 105–113 (1989)

AbouZid S. Silymarin, Natural Flavonolignans from Milk Thistle. In: Venketeshwer R, editor. Phytochemicals-A Global Perspective of Their Role in Nutrition and Health. Rijeka: Croatia InTech 11, 255–272 (2012)

Surai PF. Silymarin as a Natural Antioxidant: An Overview of the Current Evidence and Perspectives. Antioxidants (Basel). 20, 204-47 (2015)

Zhao J, Agarwal R. Tissue distribution of silibinin the major active constituent of silymarin in mice and its association with enhancement of phase II enzymes: Implications in cancer chemoprevention. Carcinogenesis, 20, 2101–2108 (1999)

Saller R, Melzer J, Reichling J, Brignoli R, Meier R. An updated systematic review of the pharmacology of silymarin. Forsch Komp Klas Nat,14, 70–80 (2007)

Krishnappa P, Venkatarangaiah K, Shivamogga V, Rajanna S.K, Prakash K, Gupta R. Antioxidant and prophylactic effects of Delonix elata L. stem bark extracts, and flavonoid isolated quercetin against carbon tetrachloride-induced hepatotoxicity in rats. Biomed. Res. Int. 2-14(2014)

Shine V.J., Latha P.G., Suja S.N., Anuja G.I., Raj G., Rajasekharan S.N. Ameliorative effect of alkaloid extract of Cyclea peltata (Poir.) Hook. f. & Thoms. Roots (ACP) on APAP/CCl4 induced liver toxicity in Wistar rats and in vitro free radical scavenging property. Asian Pac. J. Trop. Biomed,4, 143–151 (2014)

P. C. Sharma, V. Bhatia, N. Bansal, and A. Sharma, “A review on bael tree,” Indian Journal of Natural Products and Resources (IJNPR), 6, 171–178, (2007)

C. K. Pathirana, Assessment of the elite accessions of bael Aegle marmelos (L.) Corr. in Sri Lanka based on morphometric, organoleptic, and elemental properties of the fruits and phylogenetic relationships. PLoS One, 15, (2020)

Monika S, Thirumal M, Kumar PR. Phytochemical and biological review of Aegle marmelos Linn. Future Sci OA. 23, 0849-0856 (2023)

Suntar I. Importance of ethnopharmacological studies in drug discovery: role of medicinal plants. Phytochem. Rev, 19, 1199–1209 (2020)

Ahmad W, Amir M, Ahmad A et al. Aegle marmelos leaf extracts phytochemical analysis, cytotoxicity, in vitro antioxidant and antidiabetic activities. Plants, 10, 2573-2585 (2021)

Subramaniam D, Giridharan P, Murmu N et al. Activation of Apoptosis by 1-Hydroxy-5,7-Dimethoxy-2-Naphthalene-Carboxaldehyde, a Novel Compound from Aegle marmelos. Cancer Res. 20, 8573–8581 (2008)

Panth N, Paudel KR, Parajuli K. Reactive Oxygen Species: A Key Hallmark of Cardiovascular Disease. Adv. Med. 10, 1–12 (2016)

Sihombing JR, Dharma A, Chaidir Z, Almahdy A, Fachrial E, and Munaf E. Phytochemical Screening and Antioxidant Activities of 31 Fruit Peel Extract from Sumatera, Indonesia. Journal of Chemical and Pharmaceutical Research. 11, 190-6 (2015)

Reitman, S., Frankel, S. A colorimetric method for determination of serum glutamate oxaloacetate and glutamic pyruvate transaminase. American Journal of Clinical Pathology, 28, 56–58 (1957)

Varley H, Alan HG. Tests in renal disease. In: Practical Clinical Biochemistry. Vol 1123, William Heinemann Medical Book Ltd., London. 1123-1130 (1984)

Braun HP, Deneke U, Rittersdorf W. Analytical performance of reflotroncreatin kinase reagent carriers compared with the CK NAC-method. Clin. Chem,33, 988-995 (1987)

Kirtane AJ, Leder DM, Waikar SS, Chertow GM, Ray KK, Pinto DS, Karmpaliotis D, Burger AJ, Murphy SA, Cannon CP, Braunwald E, Gibson CM; TIMI Study Group. Serum blood urea nitrogen as an independent marker of subsequent mortality among patients with acute coronary syndromes and normal to mildly reduced glomerular filtration rates. J. Am. Coll. Cardiol, 45,1781–1786 (2005)

Pocock G, Richards CD.Human physiology. The basis of Medicine (3rd ed). Oxford University Press (2006)

Adelman RD, Spangler WL, Beasom F, Ishizaki G, Conzelman GM. Frusemide enhancement of neltimicin nephrotoxicity in dogs. Journal of Antimicrobial Chemotherapy, 7 431–440 (2016)

Giannini EG, Testa R, Savarino V. Liver enzyme alteration: a guide for clinicians. CMAJ, 172, 367-79 (2010)

Bouhrim M, Ouassou H, Choukri M, Mekhfi H, Ziyyat A, Legssyer A, et al. Hepatoprotective effect of Opuntia dillenii seed oil on CCl4 induced acute liver damage in rat. Asian Pac J Trop Biomed, 5, 254-262 (2018)

Lee GH, Lee HY, Choi MK, Chung HW, Kim SW, Chae HJ. Protective effect of Curcuma longa L. extract on CCl4-induced acute hepatic stress. BMC Res Notes, 10, 77-78 (2017)

Chowdhury M, De PK, Maji HS, Das D. Safety study of carboxymethylated Basella alba mucilage: a subchronic oral toxicity evaluation in Wistar albino rats. J. Appl. Pharm. Res., 12, 16–26 (2024).

Yang CL, Lin YS, Liu KF, Peng WH, Hsu CM. Hepatoprotective Mechanisms of Taxifolin on Carbon Tetrachloride-Induced Acute Liver Injury in Mice. Nutrients, 11, 2655 -2664(2019)