Innovative and cost-effective SesZen-Bio™ with enriched Biotin and improved superior dissolution efficiency

Mihir Gadani; Sneha Badak; Ratna Upadhyay

doi:10.69857/joapr.v12i3.446

Authors

Mihir Gadani Department of Phytochemistry, Zenherbs Lab Pvt Ltd, Mumbai, India
Sneha Badak Department of Phytochemistry, Zenherbs Lab Pvt Ltd, Mumbai, India
Ratna Upadhyay Department of Phytochemistry, Zenherbs Lab Pvt Ltd, Mumbai, India

DOI:

https://doi.org/10.69857/joapr.v12i3.446

Keywords:

Biotin, nutrition, natural, standardized, SesZen-BioTM

Abstract

Background: Micronutrient deficiencies, including biotin, iron, zinc, and vitamin D, can lead to hair problems such as loss and thinning. This study explores the improvement of SesZen-Bio^TM, a natural biotin supplement derived from Sesbania grandiflora leaves, to give NI- SesZen-Bio^TM. Materials: The nature and structure of biotin in SesZen-Bio^TM were evaluated using C14 analysis and ¹H NMR. Further, Sesbania grandiflora was subjected to an improved economic process to give NI-SesZen-Bio^TM. HPLTC-aided phytochemical profiling was performed for SesZen-Bio^TM, NI- SesZen-Bio^TM, and standard S. grandiflora leaf extract. The purity of d-biotin was quantified using HPLC. The dissolution profile of SesZen-Bio^TMand NI- SesZen-Bio^TMwas tested to determine bioequivalence and compared versus synthetic biotin. Results: NMR and C14 analysis revealed that SesZen-Bio^TMcontained 100% natural d-biotin. The yield for SesZen-Bio^TMand NI- SesZen-Bio^TMwas found to be 17% and 18%, respectively, and both exhibited phytochemical profiles similar to standard extract. The release rate for SesZen-Bio^TM was similar till a dose of 500 mg and slowly decreased at higher concentrations (800-100mg). A 350 mg NI- SesZen-Bio^TM showed bioequivalence to 250 mg and 500 mg for SesZen-Bio^TM. Percent d-biotin content was 0.5% in SesZen-Bio^TMand 0.81% in NI- SesZen-Bio^TM. Conclusion: This new economically driven process yielded NI-SesZen-Bio^TM, with similar yield, higher biotin (100%natural) content, and similar dissolution profile but at a reduced dosage as compared to SesZen-Bio^TMand surpassed synthetic biotin with its sustained release format. Thus, NI- SesZen-Bio^TM is a promising solution for individuals seeking natural supplements for maintaining hair and skin heath.

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