Preparation of herbal hair oil exploring the therapeutic benefits of herbs and its evaluation


  • Dhiraj Baishya Faculty of Pharmaceutical Science, Assam down town University Panikhaiti, Guwahati-781026, Assam, India
  • Ananta Choudhury Faculty of Pharmaceutical Science, Assam down town University Panikhaiti, Guwahati-781026, Assam, India
  • Himangshu Deka Faculty of Pharmaceutical Science, Assam down town University Panikhaiti, Guwahati-781026, Assam, India
  • Nurjamal Hoque Faculty of Pharmaceutical Science, Assam down town University Panikhaiti, Guwahati-781026, Assam, India
  • Rosamund Jyrwa Pratiksha Institute of Pharmaceutical Studies Chandrapur road, Panikhaiti, Guwahati-781026, Assam, India
  • Jahnabi Sarmah NEPEDS College of Pharmaceutical Sciences Tetelia, Sonapur, Guwahati-782403, Assam, India



Herbal Cosmetics, Herbal hair oil, Virgin coconut oil, Phytochemical screening, In-vitro study, In-vivo study


Background: Herbal cosmetics, particularly herbal hair oils, have gained popularity due to their perceived efficacy and minimal adverse effects. This study aimed to formulate and evaluate herbal hair oils for promoting hair growth. Methodology: Various herbal extracts, including Coconut, Curry leaves, Amla, Fenugreek, and Onion, were prepared and incorporated into virgin Coconut oil. Phytochemical screening, total flavonoid content, organoleptic evaluation, chemical analysis (acid value, saponification value, specific gravity), stability studies, antioxidant assay (DPPH), antimicrobial assay, sensitivity studies, and in-vivo hair growth activity were conducted. Results: Organoleptic evaluation revealed characteristic odors and colors across formulations. Chemical analysis showed acceptable values for acid value (0.68 - 1.86 mg KOH/g), saponification value (117.87 - 224.27 mg KOH/g), and specific gravity (0.865 - 0.933 g/cm^3). Stability studies over 45 days demonstrated consistent physicochemical properties. The DPPH assay indicated dose-dependent antioxidant activity, with inhibition ranging from 13.8% to 66.5%. Antimicrobial assay showed inhibition zones ranging from 6 mm to 20 mm against Staphylococcus aureus. Sensitivity studies exhibited no adverse reactions. In-vivo hair growth activity demonstrated significant improvements compared to controls. Discussion: The formulated herbal hair oils exhibited promising physicochemical properties, antioxidant activity, antimicrobial efficacy, safety, and hair growth promotion in animal models. These findings suggest their potential as natural remedies for hair care. Conclusion: Herbal hair oils formulated in this study show significant potential for promoting hair growth and addressing hair-related concerns. Whereas, formulation F3 shown significant efficacy across multiple parameters among the formulations. Further research and clinical trials are warranted to validate their efficacy and safety for human use.


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Rodrigues F, de la Luz Cádiz-Gurrea M, Nunes MA, Pinto D, Vinha AF, Linares IB, Oliveira MB, Carretero AS. Cosmetics. In Polyphenols: properties, recovery, and applications, Woodhead Publishing, 393-427 (2018)

Saraf S, Jharaniya M, Gupta A, Jain V, Saraf S. Herbal hair oil cosmetics: Advancements and recent findings. World J Pharm Res., 3, 3278-3294 (2014).

Marvin S. Balsam; Edward Sagarin; Cosmetics, science and technology; New York- Wiley Interscience; 2(2) (2012).

Semalty M, Semalty A, Joshi GP, Rawat MM. Hair growth and rejuvenation: an overview. Journal of dermatological treatment, 22(3), 123-32 (2011)

Kuber BR, Lavanya C, Haritha C, Preethi S, Rosa G. Preparation and evaluation of poly herbal hair oil. Journal of drug delivery and therapeutics, 9(1), 68-73 (2019).

Kadam VS, Chintale AG, Deshmukh KP, Nalwad DN. Cosmeceuticals an emerging concept: A comprehensive Review. International Journal of Research in Pharmacy and Chemistry, 3(2), 308-316 (2013).

Suzuki D. The “dirty dozen” ingredients investigated in the davidsuzuki foundation survey of chemicals in cosmetics.

Basmatker G, Jais N, Daud F. Aloe vera: a valuable multifunctional cosmetic ingredient. Int. J. Med. Aromat Plants, 1, 338-341 (2011).

No Preservatives, No Additives - Make Pure Coconut Oil At Home With Our Step-By-Step Guide;, cited September 25, 2023.

Babatunde DE, Otusemade GO, Ojewumi ME, Agboola O, Oyeniyi E. Antimicrobial activity and phytochemical screening of neem leaves and lemon grass essential oil extracts. International Journal of Mechanical Engineering and Technology,10(3), 882-889 (2019).

Begum R, Begum A. Preparation and evaluation of herbal hair oil. International Journal of Research and Analytical Reviews, 6(1), 266-269 (2019).

Jagatap NN. Formulation and Evaluation of Polyherbal Oil. International Journal of Scientific Research in Science and Technology, 8(4), 690-697 (2021).

Kolhe S, Indore P, Shewale S, Pol A, Hanmante L. Formulation and evaluation of polyherbal hair oil for alopecia. Journal of Drug Delivery and Therapeutics. 9(2-s), 387-90 (2019).

Jadhav AK, Surwase US, Thengal AV. Formulation and Evaluation of Polyherbal Hair Oil. International Journal of Science and Research, 8(10), 1250-1253 (2019).

Lahare RP, Yadav HS, Bisen YK, Dashahre AK. Estimation of total phenol, flavonoid, tannin and alkaloid content in different extracts of catharanthus roseus from Durg District, Chhattisgarh, India. Scholars Bulletin, 7(1), 1-6 (2021).

Gosavi N, Chaudhari DD, Jagdale DE, Jaiswal NR. Formulation and Evaluation of Polyherbal Lotus Oil. Research Journal of Topical and Cosmetic Sciences, 14(1), 29-44 (2023).

ICH harmonised tripartite guideline, stability testing of new drug substances and products Q1a (R2).1-24,(2003).

Carson CF, Riley TV. Antimicrobial activity of the major components of the essential oil of Melaleuca alternifolia. Journal of Applied Bacteriology, 78, 264-269 (1995).

Prabuseenivasan S, Jayakumar M, Ignacimuthu S. In vitro antibacterial activity of some plant essential oils. BMC Complementary and Alternative Medicine, 6(39), 1-8 (2006).

OECD guideline for testing of chemicals- 406 (Skin sensitisation).1-9 (1992).

Bora NS, Pathak MP, Mandal S, Mazumder B, Policegoudra R, Raju PS, Chattopadhyay P. Safety assessment and toxicological profiling of a novel combinational sunprotective dermal formulation containing melatonin and pumpkin seed oil. Regulatory Toxicology and Pharmacology, 89, 1-2 (2017).

Thorat MR, Jadhav MV, Kadam JV. Development and evaluation of polyherbal formulations for hair growth-promoting activity. International Journal of PharmTech Research, 1(4), 1251-1254 (2009).

Madhunithya E, Venkatesh G, Shyamala G, Manjari V, Ramesh S, Karuppaiah A, Sankar V. Development of ethosome comprising combined herbal extracts and its effect on hair growth. Advances in traditional medicine, 21, 131-41 (2021).



How to Cite

Dhiraj Baishya, Ananta Choudhury, Himangshu Deka, Nurjamal Hoque, Rosamund Jyrwa, & Jahnabi Sarmah. (2024). Preparation of herbal hair oil exploring the therapeutic benefits of herbs and its evaluation. Journal of Applied Pharmaceutical Research, 12(1), 116-126.