Innovative approach for improved sustained delivery of metformin hydrochloride for its anti-hyperglycemic activity
DOI:
https://doi.org/10.18231/j.joapr.2024.12.1.1.9Keywords:
Metformin hydrochloride, egg albumin, polymer, in-vitro drug release, in-vivo evaluationAbstract
Metformin hydrochloride, an antidiabetic agent, is useful in reducing the blood glucose concentration in Type II diabetes. It is also finding its use as a repurposed drug. The formulations consisted of micro drug delivery systems prepared by emulsification method and were evaluated in-vitro and in-vivo. Process variables like amount of polymer, speed of agitation and stirring, presence or absence of surfactant and cross linker offered a versatile approach towards obtaining the formulation though affected physicochemical properties of formulations. Discrete, spherical, and free-flowing microspheres, in the size range and granularity of 250 to 700µ were used to control the drug release rate. Drug release was diffusion controlled as evident from the Higuchi kinetics. The physical characteristics of the formulations were reproducible. Healthy and alloxan induced hyperglycaemic male albino mice were used for in-vivo experimentation by evaluating plasma glucose level reduction and % reduction in the blood glucose level after administration of pure drug and formulations. The results indicate significant sustained fall in the blood glucose level for about 10 hrs following formulation administration as compared to the pure drug.
Downloads
References
Franklin CK, Daniel ON, Judith CD, Bright UN, Mumuni A, Paul AA, Anthony AA. Potential enhancement of metformin hydrochloride in solidified reverse micellar solution-based PEGylated lipid nanoparticles targeting therapeutic efficacy in diabetes treatment, Heliyon, 8(4), (2022).
Yahoum MM, Toumi S, Tahraoui H, Lefnaoui S, Kebir M, Amrane A, Assadi AA, Zhang J, Mouni L. Formulation and evaluation of Xanthan Gum microspheres for the sustained release of Metformin hydrochloride. Micromachines, 14, 609-624 (2023).
Lengyel M, Kállai-Szabó N, Antal V, Laki AJ, Antal I. Microparticles, microspheres, and microcapsules for advanced drug delivery. Scientia Pharmaceutica, 87(3):20 (2019).
Hariyadi DM, Pathak Y, Hendradi E, Erawati T, Hidayah I, Santos E, Formulation of Metformin-loaded alginate microspheres by ionotropic gelation aerosolization technique, Sains Malaysiana, 49(10), 2513-2525 (2020).
Kotha AA, Ahmad SU, Dewan I, Bhuiyan MA, Rahman FI, Mohamed IN, Reza S. Metformin hydrochloride loaded mucoadhesive microspheres and nanoparticles for anti-hyperglycemic and anticancer effects using factorial experimental design, Drug Design, Development and Therapy, 17, 3661-3684 (2023).
Maestrelli F, Mura P, González-Rodríguez ML, Cózar-Bernal MJ, Rabasco AM, Mannelli LC, Ghelardini C, Calcium alginate microspheres containing Metformin hydrochloride niosomes and chitosomes aimed for oral therapy of type 2 diabetes mellitus, International Journal of Pharmaceutics, 530 (1-2), 430-439 (2017).
Nandini P, Remya PN, Krithiga M, Thomas N, Gayathri H, Saraswathi TS. Evaluation and comparison of the formulations of Amlodipine-loaded bovine serum albumin and egg albumin microspheres. Journal of Hunan University Natural Sciences, 48(8), 106-119 (2021)
Ozoude CH, Azubuike CP, Ologunagba MO, Tonuewa, Igwoli. Formulation and development of metformin-loaded microspheres using Khaya senegalensis (Meliaceae) gum as co-polymer. Future Journal of Pharmaceutical Sciences, 6, 120 (2020).
Zhuo J, Zeng Q, Cai, D, Zeng X, Chen Y, Gan H, Huang X, Yao N, Huang D, Zhang C, Evaluation of type 2 diabetic mellitus animal models via interactions between insulin and mitogen activated protein kinase signaling pathways induced by a high fat and sugar diet and streptozotocin. Molecular Medicine Reports, 17, 5132-5142 (2018).
Published
How to Cite
Issue
Section
Copyright (c) 2024 M Kar Pillai, S Pillai, S Jain
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.