Evaluation of antidiabetic and antioxidant activity of methanolic extract of Limonia acidissima leaves in alloxan-induced diabetic wister albino rats: a randomized controlled experimental study

Ashishkumar Baheti; Leena Chimurkar; Kanchan Mohod

doi:10.18231/j.joapr.2023.11.5.15.25

Authors

Ashishkumar Baheti Department of Pharmacology, Sri Aurobindo Medical College and Postgraduate Institute, Indore, Madhya Pradesh 453555
Leena Chimurkar Department of Pharmacology, Mahatma Gandhi Institute of Medical Sciences, Sewagram, Wardha, Maharashtra 442102
Kanchan Mohod Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sewagram, Wardha, Maharashtra 442102

DOI:

https://doi.org/10.18231/j.joapr.2023.11.5.15.25

Keywords:

Limonia acidissima leaves, antidiabetic, alloxan, antioxidant, histopathology, experimental study

Abstract

Objectives: The present study was carried out to find graded-doses antidiabetic and antioxidant activities of methanolic extract of Limonia acidissima (MELA) leaves in rats. Materials and Methods: Male Wistar rats were randomly divided into 6 groups (n=8): Normal control, Diabetic control (Alloxan-induced), and test groups where varying doses of MELA (100mg/kg, 200mg/kg, and 400mg/kg) or Metformin (100mg/kg) administered post-alloxan induction. Random blood sugar (RBS) levels were estimated at specific intervals over a 28-day period. Moreover, antioxidant markers (malondialdehyde, superoxide dismutase, and reduced glutathione) were assessed on day 28. Histopathological examination of pancreatic tissue was conducted. Results: Overall, mean RBS levels decreased at all intervals. There was a significant decrease in median malondialdehyde, an increase in median superoxide dismutase, and reduced glutathione levels (all p-values<0.0001). The change in RBS (p-values<0.0001) and antioxidants (p-value>0.05) levels was greater in Group VI than in Group V. On histopathology, Groups VI and V demonstrated regeneration of necrotic β-cells in the islets, and this was more pronounced than Group IV. Conclusion: Results of our study suggested that MELA possesses dose-dependent significant antidiabetic and antioxidant activity.

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References

Hameed I, Masoodi SR, Mir SA, Nabi M, Ghazanfar K, Ganai Iqra Hameed BA, Ganai BA. Type 2 diabetes mellitus: From a metabolic disorder to an inflammatory condition. World J Diabetes, 6, 598–612 (2015).

Williams Rhys, Colagiuri S, Reem A, Aschner MP, Abdul B, David B. IDF DIABETES ATLAS. 9th ed., International Diabetes Federation, (2019).

Marín-Peñalver JJ, Martín-Timón I, Sevillano-Collantes C, Javier Del Cañizo-Gómez F. Update on the treatment of type 2 diabetes mellitus. World J Diabetes, 7, 354–95 (2016).

Sena CM, Bento CF, Pereira P, Seiça R. Diabetes mellitus: new challenges and innovative therapies. EPMA J, 1, 138–63 (2010).

Cannon A, Handelsman Y, Heile M, Shannon M. Burden of Illness in Type 2 Diabetes Mellitus. J Manag Care Spec Pharm, 24, S5–13 (2018).

Fitch K, Pyenson BS, Iwasaki K. Medical Claim Cost Impact of Improved Diabetes Control for Medicare and Commercially Insured Patients with Type 2 Diabetes. JMCP Journal of Managed Care Pharmacy, 19, (2013).

Hazel-Fernandez L, Li Y, Nero D, Moretz C, Slabaugh ; S Lane, Meah Y, Baltz J, Patel NC, Bouchard JR. Relationship of Diabetes Complications Severity to Healthcare Utilization and Costs Among Medicare Advantage Beneficiaries. Am J Manag Care, 21, e62-70 (2015).

Almeida C, Rocha C, Cruz R. Impact of Adverse Events Related to Oral Antidiabetics Agents on Adherence and Quality of Life on Type 2 Diabetic Patients Diabetes & its Complications. Diabetes Complications, 4, 1–6 (2020).

Moghissi E. Management of type 2 diabetes mellitus in older patients: Current and emerging treatment options, Springer Healthcare, 2013.

Bindu J, Narendhirakannan RT. Role of medicinal plants in the management of diabetes mellitus: a review, Springer Verlag, 2019.

Rizvi SI, Mishra N. Traditional Indian medicines used for the management of diabetes mellitus. J Diabetes Res, 2013, 1–11 (2013).

Sujitha S. Insights into the Invitro Antioxidant, Anti-Inflammatory and Anticancer Activities of Limonia acidissima Fruits. Int J Life Sci Pharma Res, 11, 1–11 (2021).

Gupta R, Johri S, Saxena AM. Effect of ethanolic extract of Feronia elephantum Correa fruits on blood glucose level in normal and streptozotocin-induced diabetic rats. Natural Product Radiance, 8, 32–6 (2009).

Ilango K, Chitra V. Antidiabetic and antioxidant activity of Limonia acidissima linn. in alloxan induced rats. Pharm Lett, 1, 117–25 (2009).

Priya Darsini DT, Maheshu V, Vishnupriya M, Nishaa S, Sasikumar JM. Antioxidant potential and amino acid analysis of underutilized tropical fruit Limonia acidissima L. Free Radicals and Antioxidants, 3, S62–9 (2013).

Joshi RK, Patil PA, Muzawar MHK, Kumar D, Kholkute SD. Hypoglycemic activity of aqueous leaf extract of Feronia elephantum in normal and streptozotocin-induced diabetic rats. Pharmacologyonline, 3, 815–21 (2009).

Zygler A, Siominska M, Namiesnik J. 2.04 – Soxhlet Extraction and New Developments Such as Soxtec. Comprehensive Sampling and Sample Preparation: Analytical Techniques for Scientists, 2, 65–9 (2012).

OECD/OCDE 425 OECD GUIDELINE FOR TESTING OF CHEMICALS Acute Oral Toxicity-Up-and-Down Procedure, 2001.

Gupta SK. Antidiabetic agents. Drug Screening Methods. 3rd ed. Chap. 40, Jaypee Brothers Medical Publisher(P) Ltd, New Delhi, pp.613–6 (2016).

Hirst JA, Howick J, Aronson JK, Roberts N, Perera R, Koshiaris C, Heneghan C. The need for randomization in animal trials: An overview of systematic reviews. PLoS One, 9, 1–11 (2014).

Buege JA, Aust SD. Microsomal Lipid Peroxidation. Methods Enzymol, 52, 306–7 (1978).

Marklund S, Marklund G. Involvement of the Superoxide Anion Radical in the Autoxidation of Pyrogallol and a Convenient Assay for Superoxide Dismutase. Eur J Biochem, 47, 469–74 (1974).

Beutler E, Duron O, Kelly BM. Improved Methods for Determination of Blood Gluthatione. Journal of Laboratory Clinics Medicine, 61, 882–8 (1963).

Falkeholm L, Grant CA, Magnusson A, Möller E. Xylene-free method for histological preparation: A multicentre evaluation. Laboratory Investigation, 81, 1213–21 (2001).

Szkudelski T. The Mechanism of Alloxan and Streptozotocin Action in B Cells of the Rat Pancreas. Physiol Res, 50, 537–46 (2001).

Sarkar S, Srivastava V, Mohanty M. Statistics. Postgraduate Pharmacology. 1st ed. Chap. 6, Paras Medical Publisher, pp.375–85 (2020).

Ahmed N, Babaei-Jadidi R, Howell SK, Thornalley PJ, Beisswenger PJ. Glycated and Oxidized Protein Degradation Products Are Indicators of Fasting and Postprandial Hyperglycemia in Diabetes. Diabetes Care, 28, (2005).

Thomas A, Ponnammal NR. Preliminary studies on phytochemical and antibacterial activity of Limonia acidissima l. plant parts. Anc Sci Life, 25, 57–61 (2005).

Attarde DL, Chaudhari B, Bhambar R. Phytochemical investigation and in vitro antioxidant activity of extracts from leaves of Limonia acidissima linn. (Rutaceae). J Pharm Res, 4, 766–8 (2011).

Merinal S, Viji Stella Boi G. In vitro antioxidant activity and total phenolic content of leaf extracts of Limonia crenulata (Roxb.). Journal of natue product plant resources, 2, 209–14 (2012).

Kumawat B kumar, Chand T, Singh Y. Antidiabetic and Antihyperlipidemic Effects of Alcoholic and Aqueous Stem Bark Extracts of Limonia Acidissima, Linn in Alloxan Induced Diabetic Rats. International Journal of Phytomedicine, 4, 187–96 (2012).

Yu BP. Cellular defenses against damage from reactive oxygen species. Physiol Rev, 74, 139–62 (1994).

Ilango K, Chitra V. Hepatoprotective and antioxidant activities of fruit pulp of Limonia acidissima linn. International Journal of Health Research, 2, 361–7 (2009).