Preparation and evaluation of adipic dihydrazide cross-linked hyaluronic acid microspheres for cephalexin

B. Padmasri; R. Kalyani; V. Anilkumar; D. Prasanth; M. Indu

doi:10.18231/JOAPR.2021.9.1.30.36

Authors

B. Padmasri Dept. of Pharmaceutical Technology, Sri Venkateswara College of Pharmacy, Etcherla, Srikakulam
R. Kalyani Dept. of Pharmaceutical Technology, Sri Venkateswara College of Pharmacy, Etcherla, Srikakulam.
V. Anilkumar Dept.of Pharmaceutical Technoligy, GIET School of Pharmacy,Rajahmundry, East Godavari https://orcid.org/0000-0002-6506-2144
D. Prasanth Dept. of Pharmcology, Sri Venkateswara College of Pharmacy, Etcherla, Srikakulam
M. Indu Dept. of Pharmaceutical Technology, Sri Venkateswara College of Pharmacy, Etcherla, Srikakulam

DOI:

https://doi.org/10.18231/JOAPR.2021.9.1.30.36

Keywords:

Hyaluronic acid, microspheres, adipic acid, EDCI, Adipicdihydrazide

Abstract

Hyaluronic acid also called as Hyaluronan, Sodium Hyaluronate (SA), sodium salt form of Hyaluronic acid is a biodegradable, biocompatible, and viscoelastic linear polysaccharide of a wide molecular weight range (1000 to 10,000,000 Da). In this project, described a method for preparing HA microspheres at different pH conditions by adapting a non-toxic and aqueous based crosslinking chemistry for sustained drug delivery of drugs. The derivatization chemistry of HA utilizing adipic dihydrazide has been used to construct hydrogels, applied for microsphere preparation. ADH was coupled efficiently to carbodimide-activated glucoronic acid residues of hyluronans. These ADH modified hyaluronan can be loaded with drug molecules and then cross linked into hydrogel. The drug was present in the bulk of hydrogel droplets which are present in liquid paraffin are precipitated by IPA. Formulating HA microspheres with this method have several advantages. Preliminary studies were conducted to confirm the better ratio of HA and ADH to show maximum entrapment efficiency and drug release. Then microspheres were prepared at different pH conditions and formulations were subjected to evaluation of various parameters like percentage yield, particle size, drug entrapment efficiency, porosity and bulk density, surface morphology, in vitro drug release among which F2B was optimized as best formulation which showed 74.6% entrapment efficiency and above 90% of drug release in 12 hours indicating Hyaluronic acid microspheres can be used as good carriers for sustained drug delivery of drugs.

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References

Ramadevi K, Beula SJ, Bathula R, Suhasini GE, Nirmala M. Formulation and evaluation of microspheres of cefidinir. Asian Journal of Pharmaceutical Research, 3(2), 75-79 (2013)

Joshi JR, Patel RP. Role of biodegradable polymers in drug delivery. International Journal of Current Pharmaceutical Research, 4(4), 74-81 (2012)

Yuna H, Goetzb DJ, Paige Y, Weiliam C. Hyaluronan microspheres for sustained gene delivery and site-specific targeting. Biomaterials, 25(1), 147-57, (2004)

Goodman and Gilman's The Pharmacological Basis of Therapeutics, Pergamon Press, New York, pp. 1086-1089 (1990).

Semalthy M, Verma D, Semalthy A. Development and evaluation of microcapsules of Verapamil Hydrochloride. Indian Drugs, 48(8), 33-38, (2011).

Prashant P, Santosh S, Janardhan S. preparation and evaluation of microspheres of flurbiprofen. International Journal of Pharmaceutical Science, 9(12), 5388-5393 (2018)

Saranraj P, Naidu MA. Hyaluronic acid production and its applications- A review. International Journal of Pharmaceutical and Biological Archives, 4(5), 853-859 (2013)

Deshmukh PPM, Barhate AN. Formulation and evaluation of microspheres of Glibenclamide by inotropic gelation method. Indo American Journal of Pharmaceutical Research, 7(9), (2017)

Arianna F, Marchetti F, Pozzoli M, Raffaello U, Citemesi, Manfredini S, Vertuani S. Formulaltion and characterization of Native and Crosslilnked Hyaluronic Acid Microspheres for dermal delivery of Sodium Ascorbyl Phosphate: A Comparative study. Pharmaceutics, 10, 254, (2018)

Harsh B, Simar PK, Gupta AK. Microsphere: Methods of preparation and application; A comparative study. International Journal of Pharmaceutical Sciences Review and Research, 10(1), 69-78 (2011)

Sudhakar P, Bhagyamma SN, Siraj S, Sekhanath KV, Chowdoji Rao K, Subha MCS. Preparation and characterisation of microspheres for controlled release of anti HIV drugs. Journal of Applied Pharmaceutical Sciences, 5(2), 051-057 (2015)

Rajput S, Agarwal P, Pathak A, Nikhil S, Baghel SS, Baghel RS. A review on microspheres methods of preparation and evaluation. World Journal of Pharmacy and Pharmaceutical Sciences, 1(1), 422-438 (2012)

Kaurav H, Harikumar SL, Kaur A. Mucoadhesive Microspheres as carriers in Drug delivery: a Review. International Journal of Drug Development and Research, 4(2), 21 (2012)

Zhou Z, Siliang H, Huang T, Peng C, Zhou H, Liu Q, Zeng W, Liu L, Huang H, Xiang L, Yan H. Preparation of gelatin/hyaluronic acid microspheres with different morphologies for drug delivery. Polymer Bulletin, 72(4), 713–723, (2015)

Huh Y, Cho HJ, Yoon IS, Choi MK, Kim JS, Oh E, Chung SJ, Shim CK, Kim DD. Preparation and evaluation of spray-dried hyaluronic acid microspheres for intranasal delivery of fexofenadine hydrochloride. European Journal of Pharmaceutical Sciences, 40(1), 9-15, 2010

Chanda R, Roy A, Bahadur S, Saha S, Das S, Choudhury A. Formulation of terbutaline sulphate mucoadhesive sustained release oral tablets from natural materials and in vitro-in vivo evaluation. Asian Journal of Pharmaceutical Sciences, 5(4), 168-174 (2010)