A review of wound healing activity on different wound models

Renuka Verma; Pushpa Prasad Gupta; Trilochan Satapathy; Amit Roy

doi:10.18231/2348-0335.2018.0013

Authors

Renuka Verma Columbia Institute of Pharmacy, Vill. Tekari, Near Vidhan Sabha, Raipur (CG) 493111
Pushpa Prasad Gupta Columbia Institute of Pharmacy, Vill. Tekari, Near Vidhan Sabha, Raipur (CG) 493111
Trilochan Satapathy Columbia Institute of Pharmacy, Vill. Tekari, Near Vidhan Sabha, Raipur (CG) 493111
Amit Roy Columbia Institute of Pharmacy, Vill. Tekari, Near Vidhan Sabha, Raipur (CG) 493111

DOI:

https://doi.org/10.18231/2348-0335.2018.0013

Keywords:

Wound healing, in-vitro, ex-vivo, in-vivo, proliferation

Abstract

Wound is an injury of living tissue or break in the epithelial integrity of the upper layer of skin. This may lead to disturbance of skin anatomical structure and their function. The normal wounds start to heal immediately after an injury. Normal healing process involved: inflammatory phase, proliferation phase and maturation phase. Now a day wound healing is a challenging clinical problem. So, necessity of effective wound management is required. The various screening models play important role to understand the basic process of tissue repair and treatment of wounds. Wide varieties of in-vitro, ex-vivo and in-vivo models have been developed for evaluation of wound healing activity. The in-vitro models includes Chick chorioallantoic membrane assay, Fibroblast assay, Collagen assay, Scratch assay, Endothelial cell in vitro tube formation assay, Keratinocytes assay. Ex-vivo models includes organotypic culture, human ex vivo skin culture, porcine model, Human organotypic skin explanted culture and the in-vivo models includes Excision wound model, Incision wound Model, Burn wound model, Dead space wound model.The aim of this review article is detailed study of different types of in-vitro, ex-vivo and in-vivo models for evaluation of wound healing activity.

Downloads

Download data is not yet available.

References

Rawat S, Singh R, Thakur P, Kaur S, Semwall A .Wound healing agents from medicinal plants: A review. Asian Pacific Journal of Tropical Biomedicine, 2 , 1910¬-1917 (2012)

Perelman S, Francis G, Rutledge T, Foote J, Martino F, Dranitsaris G. Sterile Versus Nonsterile Gloves for Repair of Uncomplicated Lacerations in the Emergency Department: A Randomized Controlled Trial. Annals of Emergency Medicine, 3, 362-370 (2004)

Velnar T, Bailey T , Smrkolj V, The Wound Healing Process: an Overview of the Cellular and Molecular Mechanisms. The Journal of International Medical Research, 37, 1528 -1542 (2009)

Wang P, Huang B, Horng H, Yeh C, Chen Y. Wound healing. Journal of the Chinese Medical Association,81,94-101(2018)

Hanna R, DPM, Giacopelli J, DPM, MS, FACFAS. A Review of Wound Healing and Wound Dressing Products. Journal of Foot and Ankle Surgery, 36, 2-14 (1997)

Kumar V, Khan A, Nagarajan K. Animal Models for the Evaluation of Wound Healing Activity. International Bulletin of Drug Research, 3, 93-107 (2013)

Hunt T.K, Hopf H, Hussain Z. Physiology of Wound Healing. Advances in Skin & Wound Care,13, 6-11(2000)

Reibman J, Meixler S , Lee T.C , Gold L.I , Cronstein B N , Haines K A . Transforming Growth Factor Beta 1, A Potent Chemoattractant for Human Neutrophils, Bypasses Classic Signal-Transduction Pathways. Proceedings of the National Academy of Sciences of the United States of America, 88, 6805-6809 (1991)

Fisher C , Gilbertson-Beadling S , Powers E.A , Petzold G , Poorman R, Mitchell M.A . Interstitial Collagenase is Required for Angiogenesis in Vitro. Developmental Biology, 162, 499-510 (1995)

Shay J.W, Wright W.E . The use of Telomerized Cells for Tissue Engineering. Natural Biotechnology, 18, 22-23 (2000)

Subalakshmi M, Saranya A, Uma maheswari M, Jarina A, Kavimani S, Murali R .An Overview of the Current Methodologies used for the Evaluation of Drugs Having Wound Healing Activity. International Journal of Experimental Pharmacology,4, 127-131 (2014)

Shukla A, rasik A, jain G, Shankar R, kulshrestha D, dhawan B. in Vitro and in Vivo Wound Healing Activity of Asiaticoside Isolated from Centella Asiatica. Journal of ethnopharmacology, 65, 1-11 (1999)

Roberts A, Sporn M, Assoian R, Smith J, Roche N,Wakefield L. Transforming Growth Factor Type and Rapid Induction of Fibrosis and Angiogenesis in Vivo and Stimulation of Collagen Formation in Vitro. Proceeding of the National Academy of Sciences of the United States of America,83, 4167-4171 (2000)

Brown L.F, Yeo K, Berse B, Yeo T, Donald R, Senger D, Dvorak F, Vandewater D. Expression of Vascular Permeability Factor (Vascular Endothelial Growth Factor) by Epidermal Keratinocytes during Wound Healing. Journal of Experimental Medicine, 176, 1375-1379 (1992)

Chereddy k, Coco R, Memvanga P, Ucakar B, Rieux A.D, Vandermeulen G, Preat V. Combined Effect of PLGA and Curcumin on Wound Healing Activity. Journal of Controlled Release,171, 208-215 (2013)

Liang C, Park A, Guan J. In Vitro Scratch Assay: a Convenient and Inexpensive Method for Analysis of Cell Migration in Vitro. Journal of Natural Protocols, 2, 29-33 (2007)

Montoya A, Daza A, Muñoz D, Ríos K, Taylor V, Cedeño D. Development of a Novel Formulation with Hypericin to Treat Cutaneous Leishmaniasis Based on Photodynamic Therapy in invitro and in Vivo Studies. Antimicrobial Agents and Chemotherapy, 59, 5804-5813 (2015)

Talekar Y.P , Apte K.G, Paygude S.V, Tondare P.R, Parab P.B. Studies on Wound Healing Potential of Polyherbal Formulation Using in Vitro and in Vivo Assays. Journal of Ayurveda and Integrative Medicine, 8, 73-81(2017)

Demirci S, Dogan A, DemirciS¸ ahin F. In vitro Wound Healing Activity of Methanol Extract of Verbascum Speciosum. International journal of Applied Research in Natural Product, 7, 37-44 (2014)

Koolwijk P, Vanerck M.G, DeVree W.J, Vermeer M.A, Weich H.A, Hanemaaijer R, Hinsbergh V. Cooperative Effect of TNF Alpha, bFGF, and VEGF on the Formation of Tubular Structures of Human Microvascular Endothelial Cells in a Fibrin Matrix. Role of Urokinase Activity. The Journal of Cell Biology,132, 1177-1188 (1996)

Shrivastava A, Mishra A, Alia S.S, Ahmad A, Abuzinadah M.F, Khana N.A. In Vivo Models for Assesment of Wound Healing Potential: A Systematic Review. Wound Medicine, 20, 43-53 (2018)

Keese C, Wegener J, Walker S, Giaever I. Electrical Wound-Healing Assay for Cells In Vitro. Proceeding of the National Academy of Science of the United States of America, 101, 1554–1559 (2004)

Andrade T.A, Aguiar A.F, Guedes F.A, Leite M.N, Caetano G.F, Coelho E.B, Das P.K, Frade M.A, Ex Vivo Model of Human Skin (hOSEC) as Alternative to Animal use for Cosmetic Tests. Procedia Engineering, 110, 67-73 (2015).

Frade M, Andrade T, Aguiar A, Guedes F, Leite M, Passos W, Coelho E, Das P. Prolonged Viability of Human Organotypic Skin Explant in Culture Method (hOSEC). Anias Brasileiros Dermatologia, 90, 347-50 (2015)

Flueckiger F, Kodjikian L, Halberstadt M, Boehnke M, Garweg J. an ex-vivo, Whole-globe Porcine Model of Corneoepithelial Wound Healing Tested Using Immunomodulatory Drugs. Journal of Ocular Pharmacology and Therapeutics, 21, 365-375 (2005)

Greenwald P, Gottlieb J, Mass P. Full-Thickness Skin Wound Explants in Tissue Culture: a Mechanical Evaluation of Healing. Plastic and Reconstruction Surgery, 90, 289-294 (1992)

Burd A, Longaker T, Adzick N.S, Campton C, Harrison M, Siebert J.W, Ehrlich H.P. Fetal Wound Healing: an in vitro Explant Model. Journal of Pediatric Surgery, 25, 898-901 (1990)

Kirkpatrick CJ, Krump -Konvalinkova V, Unger R E, Bittinger F, Otto M, Peters K. Tissue Response and Biomaterial Integration: the Efficacy of in vitro Methods. Biomolecular Engineering, 19, 211-217 (2002)

Xu W, Hong S, Jia S, Zhao Y, Galiano R, Mustoe T. Application of a Partial-Thickness Human ex vivo Skin Culture Model in Cutaneous Wound Healing Study. Laboratory Investigation, 92, 584-599 (2012)

Krishnaveni B, Neeharika V, Venkatesh S, Padmavathy R, Reddy B.M. Wound Healing Activity of Carallia Brachiata Bark. Indian Journal of Pharmaceutical Sciences, 71, 576-578 (2009)

Agarwal P, Singh A, Gaurav K, Goel S, Khanna H, Goel R.K . Evaluation of Wound Healing Activity of Extracts of Plantain Banana (Musa sapientum var. paradisiaca) in Rats. Indian Journal of Experimental Biology, 47, 32-40 (2009)

Patil D, Kulkarni R., Shahapurkar A, Hatappakki C. Natural Cumin Seeds for Wound Healing Activity in Albino Rats. International Journal of Biological Chemistry, 3,148-152 (2009)

Murthy S, m. K. goel G, Purohit V, Sharma H, Goel R. K. Evaluation of in vivo Wound Healing Activity of Bacopa Monniera on Different Wound Model in Rats. Biomed Research International Volume, 201, 1-9 (2013)

Singh D, Nag M, patel S, Pradhan M, shrivastava S, daharwal S. Treatment Strategies in Burn Wounds: an Overview. Research journal of Pharmacology and pharmacodynamics,5, 341-35 (2013)

Gurung S, Skalko-Basnet N. Wound Healing Properties of Carica Papaya Latex: in vivo Evaluation in Mice Burn Model. Journal of Ethnopharmacology, 121, 338-341 (2009)

Srivastava P, Durgaprasad S. Burn Wound Healing Property of Cocos Nucifera: an appraisal. Indian Journal of Pharmacology, 40, 144–146 (2008)

Azeez S, Amudhan S, Adiga S, Rao N, Udupa LA. Wound Healing Profile of Areca Catechu Extracts on Different Wound Models in Wistar Rats. Kuwait Medical Journal, 39, 48-52 (2007)

Asif A, Kakub G, Mehmood S, Khunum R, Gulfraz M. Wound Healing Activity of Root Extracts of Berberis Lyceum Royle in Rats. Phytotherapy Research, 21,589-591 (2007)

Tajik H, Jalali F.S.S. Influence of Aqueous Extract of Yarrow on Healing Process of Experimental Burn Wound in Rabbit: Clinical and Microbiological Study. Journal of Animal and Veterinary Advances, 6,1464-1468 (2007)

Eissa A.E, Zaki M.M, Saeid S, Abdelsalam M, Ali H.M, Moustafa A.A. In Vitro Evaluation of the Efficacy of Hemodialysate (Solcoseryl_) as a Wound Healing Agent in Nile Tilapia (Oreochromis niloticus). International Journal of Veterinary Science and Medicine, 1, 57-64 (2013)

Rollin, B.E. Toxicology and New Social Ethics for Animals. Toxicologic Pathology, 31,128-131 (2003)

Doke S.K, Dhawale S.C. Alternatives to Animal Testing: A Review. Saudi Pharmaceutical Journal, 23, 223-229 (2015)