Evaluation of analgesic and anti-inflammatory activity of ethanolic extract of Enhydra fluctuans on male wistar rats

Kishor Kumar Roy; Md. Kabirul Islam Mollah; Md. Masud Reja; Dibyendu  Shil; Ranjan Kumar Maji

doi:10.18231/JOAPR.2021.9.1.37.42

Authors

Kishor Kumar Roy Department of Pharmacology, Bharat Technology, Uluberia, Howrah, West Bengal
Md. Kabirul Islam Mollah Department of Pharmacology, Bharat Technology, Uluberia, Howrah, West Bengal
Md. Masud Reja Mata Gujri College of Pharmacy, Kishanganj, Bihar
Dibyendu Shil Mata Gujri College of Pharmacy, Kishanganj, Bihar
Ranjan Kumar Maji Department of Pharmacology, Bharat Technology, Uluberia, Howrah, West Bengal

DOI:

https://doi.org/10.18231/JOAPR.2021.9.1.37.42

Keywords:

Enhydra fluctuans, Eddy’s hot plate, formalin, paw edema, analgesic, anti-inflammatory

Abstract

Enhydra fluctuations an edible semi-aquatic vegetable plant are widely used in the traditional system of medicine. Ethanolic extract of Enhydra fluctuans was screened for analgesic & anti-inflammatory activity. Analgesic activity was evaluated by the hot plate method and anti-inflammatory activity was evaluated by formalin induce paw edema in Wistar rats (male). Ethanolic extract dose of 300 mg/kg reduce formalin induce paw inflammation and also increased the pain threshold in rats evidenced by hot plate method. The experimental results concluded that Enhydra fluctuans have significant analgesic and anti-inflammatory activity may due to flavonoid and phenolic compound content.

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References

Sharma U, Borah VB, Saikia KK. Enhydra fluctuans: A review on its pharmacological importance as a medicinal plant and prevalence and use in North-East India. Int. J. Pharm. Pharm. Sci., 6(2), 48-50 (2014).

Ali MR, Billah MM, Hassan MM et al. Enhydra fluctuans Lour: A Review. Res. J. Pharm. Tech., 6(9): 927-929 (2013).

Ullah MO, Haque M, Urmi KF, Zulfiker AHM, Anita ES, Begum M et al. Anti-bacterial activity and brine shrimp lethality bioassay of methanolic extracts of fourteen different edible vegetables from Bangladesh. Asian Pac. J. Trop. Biomed., 3(1), 1-7 (2013).

Krishnaswamy NR, Ramji N. Sesquiterpenes lactones from Enhydra fluctuans. J. Phytochem., 38(2), 433-435 (1995).

Amin MR, Mondol R, Hossain MT et al., Antimicrobial activity and cytoxic activity of three bitter plants Enhydra fluctuans, Andrographis paniculata and Clerodendrum viscosum. Adv. Pharm. Bulletin, 2(2), 207-211 (2012).

Dasgupta N, De B. Antioxidant activity of some leafy vegetables of India: A comparative Study. Food Chem., 101, 471-474 (2007).

Uddin SJ, Ferdous MM, Rouf R. Evaluation of Anti-diarrheal Activity of Enhydra fluctuans. Med Sc., 5 (4), 324-327 (2011).

Bhakta JN, Majumdar P, Munekage Y. Antimicrobial efficacies of methanol extract of Asteracantha longifolia, Ipomoea aquatica and Enhydra fluctuans against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Micrococcus luteus. Internet J. Alter. Med., 7(2), 125-135 (2009).

Sannigrahi S, Mazumder UK, Pal D. Kumar P, Mishra SL, Maity S. Flavonoids of Enhydra fluctuans exhibits analgesic and anti-inflammatory activity in different animal model. J. Pharm. Sci., 24(3), 369-375 (2011).

Roy SK, Mazumder U, Islam A. Pharmacological evaluatio of Enhydra fluctuans aerial parts for central nervous system depressant activity. Pharmacologyonline, 1, 632-643 (2011).

Rahman MT, Gegum N. Analgesic activity of Enhydra fluctuans. Fitoterapia, 73(7-8), 654-660 (2002).

Kumar GP, Khanum F. Neuroprotective potentials of Phytochemicals. Pharmacogn. Rev., 6(12), 81-90 (2012).

Das AK, Dutta BK, Sharma GD. Medicinal plant used by different tribes of Cachar District, Assam, Indian J. Tradt. Knowl., 7(3), 446-454 (2008).

Ganguly SN, Ganguly T, Sircar SM. Gibberelins of Enhydra fluctuans. J. Phytochem., 11, 3433-3434 (1972).

Garcia LA, Guillamon E, Villares A, Rostagno MA, Martinez JA. Flavonoids as anti-inflammatory agents: implication in cancer and cardiovascular diseases. Inflamm. Res., 58, 537-552 (2009).

Patil SK, Majumder P, Wedekar RR. Effect of Enhydra fluctuans Lour. Leaf extract on phagocytosis by human neutrophils. J. Nat. Rem. 8(1), 76-81 (2008).

Sannigrahi S, Mazumder UK, Mondal A, Pal DK, Mishra SL, Roy S. Flavonoids of Enhydra fluctuans exhibits anticancer activity against Ehrlich’s Ascites Carcinoma in mice. Nat. Prod. Commn., 5, 1239-1242 (2009).

Hazra H, Alfasana MA, Khondaker M. Biochemical compositions of some selected aquatic macrophytes, Bangladesh: Lambert Academic Publishing, 2012.

Jaiswal V. Culture and ethnobotany of Jaintia tribal community of Meghalaya North East India- A mini review. Indian J. Tradt Knowl., 9(1), 38-44 (2010).

Fan Sook-Ha, Ali NA, Basri FD, Evaluation of analgesic activity of the methanolic extract from the Galls of Quercus infectoria (Olivier) in rats. Hindawi Publishing Corporation, 1-6 (2014).

Kumar SP, Jagannath PV, Chandra DS, Prasan ND. Hepatoprotective activity of Enhydra fluctuans Lour aerial parts against CCl4 induced hepatotoxicity in rats. Inter J. Res Ayur Pharm., 3(6), 893-896 (2012).

Dibyendu S, Damiki L, Smriti RCD, Suvakanta D. Pharmacognostical And Phytochemical Standardization Of Physalis Minima L. Leaf, Int. j. Pharm Pharm Sci.,11(11), 20-25 (2019).

Kokate CK. Practical pharmacognosy, 5th edition, Vallabh prakashan, Delhi, 123-124 (2014).

Dibyendu S, Suvakanta D, Damiki L, Jashabir C, Sumit D. Evaluation of immunomodulatory effect of methanolic extract of Mirabilis jalapa L. tuber on mice. Res. J. Pharm Biol Chem Sci., 8(5), 49-58 (2017).

Yimer T, Birru EM, Adugna M, Geta M, Emiru YK. Evaluation of Analgesic and Anti-Inflammatory Activities of 80% Methanol Root Extract of Echinops kebericho M. (Asteraceae). J. Inflammation Res., 13, 647—658 (2020).

Konate K, Bassole IHN, Hilou A et al. Toxicity assessment and analgesic activity investigation of aqueous acetone extracts of Sida acuta Burn f. and Sida cordifolia L. (Malvaceae), medicinal plants of Burkina Faso. BMC Complement Altern Med., 12(1), 120 (2012).

Tadiwos Y, Nedi T, Engidawork E. Analgesic and anti-inflammatory activities of 80% methanol root extract of Jasminum abyssinicum Hochst. ex. Dc. (Oleaceae) in mice. J Ethnopharmacol., 202, 281–289 (2017).

Sun K, Song X, Jia R, et al. Evaluation of analgesic and anti-inflammatory activities of water extract of Galla chinensis in vivo models. Evid Based Complement Altern Med., 6784032 (2018).