Evaluation of analgesic, antioxidant and cytotoxic activities of ethanol extract of Clerodendrum viscosum Vent
Keywords:Clerodendrum viscosum, Analgesic activity, Antioxidant activity, Cytotoxic activity
Clerodendrum viscosum Vent. is a very common plant in Bangladesh which is locally familiar as “Bhat” or “Ghetu”. Here, the ethanol extract of whole plant part of C. viscosum and its various solvent (petroleum-ether, chloroform and ethyl acetate) fractions were subjected for the appraisal of analgesic, antioxidant and cytotoxic activities. Analgesic activity was tested by acetic acid-induced writhing model in Swiss albino mice. All the plant samples at the oral doses of 100- and 200 mg/kg body weight were found to exhibit significant (p<0.05) pain reducing activity in test animals. Highest inhibition of writhing was 62.38% by the ethyl acetate soluble fraction at dose of 200 mg/kg body weight while the standard drug diclofenac sodium (50 mg/kg) produces 76.14% reduction of abdominal writhing. DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical quenching assay was employed to determine the antioxidant potential of the plant samples while cytotoxic activity was checked by brine shrimp lethality bioassay. In DPPH radical scavenging assay, the plant samples showed prominent antioxidant activity. Among all, the ethyl acetate fraction showed maximum antioxidant potential with IC50 value of 28.02±0.53 μg/ml. In cytotoxic assay, the petroleum-ether fraction demonstrated strong cytotoxicity with LC50 value of 1.42±1.12 μg/ml. In summary, C. viscosum extracts possess significant analgesic, antioxidant and cytotoxic activities which rationalize its traditional use in folk medicine.
Newman DJ and Cragg GM. Natural products as sources of new drugs from 1981 to 2014. Journal of Natural Products, 79, 629-661 (2016).
Cragg GM and Newman DJ. Natural products: a continuing source of novel drug leads. Biochimica et Biophysica Acta, 1830, 3670-3695 (2013).
Das SC, Qais N, Kuddus MR and Hasan CM. Isolation and characterization of (22E, 24S)-Stigmasta-5, 22, 25-trien-3β-ol from Clerodendrum viscosum Vent. Asian Journal of Chemistry, 25, 6447-6448 (2013).
Azad AK, Wan Azizi WS, Syafiq TMF, Mahmood S, Almoustafa HA and Labu ZK. Isolation, characterization and cytotoxic effect exploration of ethanolic extract of local medicinal plant Clerodendrumn viscosum vent. Australian Journal of Basic and Applied Sciences, 7, 641-647 (2013).
Kuddus MR, Rumi F, Haque MR, Hassan MA and Rashid MA. Assessment of antioxidant, antimicrobial and cytotoxic properties of fruits of Melocanna baccifera (Roxb.) Kurz. Turkish Journal of Pharmaceutical Sciences, 10, 185-192 (2013).
Fahad TA, Kuddus MR and Hasan CM. Phytochemical and biological studies of bark extract of Miliusa velutina (Dunal) Hook. f. & Thomson. Dhaka University Journal of Pharmaceutical Sciences, 19, 125-131 (2020).
Anjum N, Hossain MJ, Haque MR, Chowdhury A, Rashid MA and Kuddus MR. Phytochemical investigation of Schleichera oleosa (Lour.) Oken Leaf. Bangladesh Pharmaceutical Journal, 24, 33-36 (2011).
VanWagenen BC, Larsen R, Cardellina JH, Dazzo RD, Lider ZC and Swithenbank C. Ulosantoin, a potent insecticide from the sponge Ulosa ruetzleri. Journal of Organic Chemistry, 58, 335-337 (1993).
Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain, 16, 109-110 (1983).
Ahmed F, Selim MST, Das AK and Choudhuri MSK. Anti-inflammatory and antinociceptive activities of Lippia nodiflora Linn. Pharmazie 59, 329-33 (2004).
Brand-Williams W, Cuvelier ME and Berset C. Use of free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28, 25-30 (1995).
McLaughlin JL and Rogers LL. The use of biological assays to evaluate botanicals. Drug Information Journal, 32, 513-524 (1998).
Gawade S. Acetic acid induced painful endogenous infliction in writhing test on mice. Journal of Pharmacology and Pharmacotherapeutics, 3, 348 (2012).
Akindele AJ, Ibe IF, Adeyemi OO. Analgesic and antipyretic activities of Drymaria cordata (Linn.) Willd (Caryophyllaceae) extract. African Journal of Traditional, Complementary, and Alternative Medicines, 9, 25-35 (2012).
Machioro M, Blank MFA, Moura RHV, Antioniolli AR. Antinociceptive activity of the aqueous extract of Erythrina velutina leaves. Fitoterapia, 6, 637-642 (2005).
Sumi SA, Biswas NN, Islam MK and Ali MK. Evaluation of analgesic and antioxidant properties in the ethanolic root extract of Clerodendrum viscosum Vent. International Journal of Pharma Sciences and Research, 6(5), 882-885 (2015).
Shah MAR, Khan RA and Ahme, M. Sorghum halepense (L.) Pers rhizomes inhibitory potential against diabetes and free radicals. Clinical Phytoscience, 7, 19 (2021).
Lourenço SC, Moldão-Martins M and Alves VD. Antioxidants of natural Plant Origins: From sources to food industry applications. Molecules (Basel, Switzerland), 24, 4132 (2019).
Sarah QS, Anny FC and Misbahuddin M. Brine shrimp lethality assay. Bangladesh Journal of Pharmacology, 12, 186-189 (2017).
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Copyright (c) 2021 Sujan Chandra Das, Md. Ruhul Kuddus, Nazmul Qais, Choudhury M. Hasan
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