Pharmacognostical, physiochemical and phytochemical evaluation of leaf, stem and root of orchid Dendrobium ochreatum

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Janmajoy Banerjee Neelmani Chauhan Biplab Kumar Dey


The present work was aimed to carry out pharmacognosical and phytochemical evaluation of individual root, stem, and leaves of orchid “Dendrobium ochreatum.” The plant was sun dried and was grounded to fine powder using mechanical grinder followed by sieving. The fine powder was collected and subjected to different pharmacognostical studies like fluorescence analysis under uv light at different wavelength. Physiochemical parameters were also evaluated of the dried plant parts like ash values, loss on drying. Each part of plant like root, stem and leaves were separated and subjected to extraction using soxhletion using different polarity solvents i,e hexane, chloroform, ethanol in gradient elution technique. All total nine plant extracts were obtained, phytochemical screening revealed the presence of important phytoconstituents like alkaloid, glycoside, saponins etc whereas only chloroform extracts of stem exhibited the presence of steroid/phytosterol.

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BANERJEE, Janmajoy; CHAUHAN, Neelmani; DEY, Biplab Kumar. Pharmacognostical, physiochemical and phytochemical evaluation of leaf, stem and root of orchid Dendrobium ochreatum. Journal of Applied Pharmaceutical Research, [S.l.], v. 6, n. 1, p. 16-25, apr. 2018. ISSN 2348-0335. Available at: <>. Date accessed: 23 apr. 2018. doi:


[1] Rajendra Yonzone D. Lama, R. B. Bhujel, Samuel Rai. Orchid species diversity of Darjeeling Himalaya of India, International Journal of Pharmacy & Life Sciences, 3(3): 1533-1550, (2012).
[2] Mamta Saxena, Jyoti Saxena, Rajeev Nema, Dharmendra Singh, Abhishek Gupta, Phytochemistry of Medicinal Plants, Journal of Pharmacognosy and Phytochemistry, 1(6): 168-182, (2013).
[3] Sumitra Chanda. Importance of pharmacognostic study of medicinal plants: An overview, Journal of Pharmacognosy and Phytochemistry, 2 (5): 69-73, (2014).
[4] Anonymous, Indian Pharmacopoeia, CSIR publication, V-2, A-53-54, (1996).
[5] P.C Sharma, M. B Yelne, T.J Dennis, Database on Medicinal Plants used in Ayurveda, CCRAS, Dept of ISHM& H. Ministry of H&FW. Govt of India, New Delhi. 2: 270-276, ( 1999).
[6] WHO guidelines, AITBS Publishers and Distributors: New Delhi. 1:28- 46, (2002).
[7] Anonymous, “The Pharmacopoeia of India” Controller of Publication, 4th Edition. New Delhi, A-70-74, (1996).
[8] M.Jhonson, N .Janakiram, Phytochemical and TLC studies on stem and leaves of the orchids Dendrobium Panduratu, Indian journal of Natural Products and Resources, 4(3):250-254, (2013).
[9] C.K Kokate, Practical Pharmacognosy, Vallabh Prakashan, 14: 178-182.(1994).

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