Nitrite contents in various masticatories prevailing in the state of Assam – a pilot study

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Rituraj Bharadwaj Prajjalendra Barooah Snigdha Saikia Jayanta Haloi Manab Deka Bhanu P Sahu Subhash Medhi


Background: The habit of using masticatories is quite common among the population of the state Assam. There are numerous types of masticatories available throughout the state, most of which include areca nut in different forms, accompanied with beetle leaves and tobacco leaves and other associated substances added for flavor. Aim: To determine the level of nitrite present in different types of masticatories prevailing in the state.  Materials & Methods: The classical Griess method was used for the estimation of nitrite in different types of these masticatories prevailing in the state Assam (n=35) using the basic diazotization principle and spectrophotometric determination. Statistical Analysis used: The results obtained were analyzed statistically by using SPSS V.16 software. Results: The result of the study indicate the amount of nitrite in different types of these masticatories, among which highest levels of nitrite was found in raw areca nut with beetle leaves without lime. The presence of lime decreases the amount of nitrite (p<0.05) whereas, the presence of beetle leaves and unprocessed areca nut increases the amount of nitrite (p<0.05) in the masticatories used by the people of the state. Conclusion: From the study conducted, it can be concluded that the presence of lime reduces the amount of nitrite in the masticatories, whereas the presence of beetle leaves and unprocessed areca nut contribute higher amount of nitrite in the masticatories.

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BHARADWAJ, Rituraj et al. Nitrite contents in various masticatories prevailing in the state of Assam – a pilot study. Journal of Applied Pharmaceutical Research, [S.l.], v. 5, n. 3, p. 21-26, july 2017. ISSN 2348-0335. Available at: <>. Date accessed: 25 sep. 2018. doi:


[1] Nandakumar A. Consolidated report of the population based cancer registries. National Cancer Registry Programme. Indian Council of Medical Research, 1990-96; New Delhi, India (2001).
[2] Pindborg JJ, Murti PR, Bhonsle RB, Gupta PC, Daftary DK, Mehta FS. Oral submucous fibrosis as a precancerous condition. Scand J Dent Res.92(3),224-9 (1984).
[3] Murti PR, Bhonsle RB, Gupta PC, Daftary DK, Pindborg JJ, Mehta FS. Etiology of oral submucous fibrosis with special reference to the role of areca nut chewing. J Oral Pathol Med.24(4),145-52 (1995).
[4] Murti PR, Bhonsle RB, Pindborg JJ et al. Malignant transformation rate in oral submucous fi brosis over a 17 year period. Community Dent Oral Epidemiol,13,340-341(1985).
[5] Koul PA, Koul SK, Sheikh MA, Tasleem RA, Shah A. Lung cancer in the Kashmir valley. Lung India, 27, 131- 137(2010).
[6] Somdatta P, Baridalyne N. Awareness of breast cancer in women of an urban unsettlement colony. Indian J Cancer, 45, 149-153 (2008).
[7] Sharma RG, Kumar R, Jain S, Jhajhria S, Gupta N, Gupta SK, Rawtani S, Kohli K, Prajapati L, Gupta R, Swamy N, Pathak D,Verma H, Ratnawat SS. Distribution of malignant neoplasms reported at different pathology centres and hospitals in Jaipur, Rajasthan. Indian J cancer, 46, 323-330 (2009).
[8] Gaur DS, Kishore S, Harsh M, Kusum A, Bansal R. Pattern of cancers among patients attending Himalayan Institute of Medical sciences, Dehradun. Indian J Pathol Microbiol, 49, 193-198 (2006).
[9] Prasad RR, Singh JK, Mandal M, Kumar M, Prasad SS. Profile of gall bladder cancer cases in Bihar. Indian J Med Paediatr Oncol, 26, 31-35 (2005).
[10] Sumathi B, Ramalingam S, Navaneethan U, Jayanthi V. Risk factors for gastric cancer in South India. Singapore Med J, 50, 147-150 (2009).
[11] Nair,U.J., Nair,J., Mathew,B. and Bartsch,H. Glutathione S-transferase M1 and T1 null genotypes as risk factors for oral leukoplakia in ethnic Indian betel quid/tobacco chewers. Carcinogenesis, 20, 743-748 (1999).
[12] Y.S. Lin, Y.M. Jen, B.B. Wang, J.C. Lee, B.H. Kang. Epidemiology of oral cavity cancer in Taiwan with emphasis on the role of betel nut chewing. ORL J. Otorhinolaryngol. Relat Spec, 67,230–236(2005).
[13] Hotchkiss JH. Preformed N-nitroso compounds in foods and beverages. Cancer Surv,8, 295-321 (1989).
[14] D. Pobel, E. Riboli, J. Cornee, B. Hemon & M. Guyader, Eur. J. Epidemiol, 11,67 (1995).
[15] S. D. Gangoli, P.A. Brandt, Vanden, V. J. Feron, C. Janzowsky, J. H. Koeman, G. J. A. Speijers, B. Spiegelhalder, R. Walker & J. S. Wishnok, Eur. J. Pharmacol. Environ. Toxicol. Pharm., 1,292 (1995).
[16] Fereidoon. Shahidi, Jozef. Synowiecki, Nrisinha. Sen. Color characteristics and absence of N-nitrosamines in nitrite-cured seal meat. J. Agric. Food Chem., 40 (8), 1398–1402 (1992).
[17] International Agency for Research on Cancer. Overall Evaluation of Carcinogenicity to Humans. IARC monographs. Vol. 1-82.http: //
[18] Wilczak, A., Assadi-Rad, A., Lai, H. H., Hoover, L. L., Berger R., Hunsinger, R. B., Beland, J. W., Lazzelle, L. J., Kincannon, E. G., Baker, H., and Heany, C. T. Formation of NDMA in chloraminated water coagulated with DAMAC cationic polymer. J. Am. Water Works Assoc,95,94–106 (2003).
[19] World Health Organization. N-Nitrosodimethylamine. Concise International Chemical Assessment Document 38. World Health Organization, Geneva (2002).
[20] AOAC Method 36.1 .21, in: ‘Official Methods of Analysis’, 16th Edn, (1995).
[21] Emilio García-Robledo, Alfonso Corzo, Sokratis Papaspyrou. A fast and direct spectrophotometric method for the sequential determination of nitrate and nitrite at low concentrations in small volumes. Marine Chemistry, 162,30-36 (2014)
[22] Lisa A. Ridnour, Julia E. Sim, Michael A. Hayward, David A. Wink, Sean M. Martin,Garry R. Buettner, and Douglas R. Spitz. A Spectrophotometric Method for the Direct Detection and Quantitation of Nitric Oxide, Nitrite, and Nitrate in Cell Culture Media. Analytical Biochemistry, 281, 223–229 (2000)
[23] Petra Kleinbongard, Tienush Rassaf, Andre Dejam, Stefan Kerber, Malts Kelm. Griess method for nitrite measurement of aqueous and protein-containing samples. Methods in Enzymology ,359,158-168 (2002)
[24] Sen NP: Nitrosamines and food poisoning, TU, AT. (ED.) Handbook of Natural Toxins, Vol 7, Food Poisoning, XXVI+624P. Marcel Dekker, INC.:New York, USA: Basel,Swizerland.Illus. ISBN 0-8247-8652-1; 0 (0) 575-607 (1992).