Investigation of oxidative potential of mahasudarshan churna using goat liver as in-vitro experimental model

Bibhas Pandit; Trilochan Satapathy; Sanjib Bahadur; Jyoti Dewangan

doi:10.18231/j.joapr.2019.002

Authors

Bibhas Pandit Columbia Institute of Pharmacy, Vill. Tekari, Near Vidhan Sabha, Raipur (CG) 493111
Trilochan Satapathy Columbia Institute of Pharmacy, Vill. Tekari, Near Vidhan Sabha, Raipur (CG) 493111
Sanjib Bahadur Columbia Institute of Pharmacy, Vill. Tekari, Near Vidhan Sabha, Raipur (CG) 493111
Jyoti Dewangan Columbia Institute of Pharmacy, Vill. Tekari, Near Vidhan Sabha, Raipur (CG) 493111

DOI:

https://doi.org/10.18231/j.joapr.2019.002

Keywords:

Mahasudarshan churna, ascorbic acid, malondialdehyde, nitric oxide, reduced glutathione, lipid peroxidation

Abstract

The American Medical Research Community sounded a heavy metal warning against some herbo-mineral Ayurvedic formulations from Indian System of Medicine sold in United States of America. The products were rejected by Unites States Food and Drug Administration due to the presence of high level of lead, mercury and arsenic as impurities, marked as toxicity inducers. This work has been design to investigate whether the toxicity produced by Mahasudarshan churna is due to the results of lipid peroxidation, as oxidative degradation of phospholipids is one of the causes of drug-induced toxicity. The level of malondialdehyde, reduced glutathione and nitric oxide were estimated in control, drug-treated, drug-antioxidant treated and only antioxidant-treated group at two hours and six hours of incubation time in goat liver homogenates. Ascorbic acid was used to compare the oxidative potentials of Mahasudarshan churna. The level of malondialdehyde was found to be decreased in the drug-treated, drug-antioxidant treated and only antioxidant treated group where as the level of reduced glutathione and nitric oxide increased when compared to control. Above all the Drug-antioxidant treated group showed maximum anti-oxidant properties when compared to other groups. The study was designed to investigate the lipid peroxidation induction capacity of Mahasudarshan churna as consequences of its toxicity and found that lipid peroxidation is not the contributing factor

Downloads

Download data is not yet available.

References

Rasheed PS, Shivshankar M. Evaluation of Herbo mineral formulations (bhasma): an overview. Int J Res Ayurveda Pharm. 6(3). 382-86 (2015).

Chaudhary A, Singh N. Herbo mineral formulations (rasaoushadhies) of ayurveda an amazing inheritance of ayurvedic pharmaceutics. Anc sci life. 30(1). 18-26 (2010).

Garg M, Singh G, Das S. Comparative physicochemical evaluation of a marketed herbomineral formulation: Naga bhasma. Indian J Pharm Sci. 74(6). 535-40 (2012).

Rajalakshmi P, Sudeer RG, Vadivel V, Sahayam CS, Brindha P. Analytical studies on Annabethi Chenthuram, A Siddha Herbomineral Formulation. Indian J Pharm Sci. 79(6). 987-93 (2017).

Nirali JB, Shankar MB. Recent Trends in the Usage of herbo-mineral formulations in healthcare system. Int J of Pharma Innov. 3(3). 116-29 (2013).

Edwards IR, Aronson JK. Adverse drug reactions: Definitions, diagnosis, and management. Lancet. 356(9237). 1255-9 (2000).

Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014. 1-31 (2014).

Girotti AW. Mechanisms of lipid peroxidation. J Free Radicals Biol Med. 1(2). 87-95 (1985).

Ray S, Roy K, Sengupta C. Evaluation of Protective Effects of Water Extract of Spirulina platensis (blue green algae) on Cisplatin-Induced Lipid Peroxidation. Indian J Pharm Sci. 69(3). 378-83 (2007).

Rajani M, Anandjiwala S, Bagul M, Parabia M. Evaluation of free radical scavenging activity of an ayurvedic formulation. Indian J Pharm Sci. 70(1). 31-5 (2008).

Vador N, Vador B, Hole R. Simple spectrophotometric methods for standardizing ayurvedic formulation. Indian J Pharm Sci. 74(2). 161-63 (2012).

Shrada BK, Dhanraj M. Role of Vitamin C in body health. Res J Pharma Tech. 11(4): 1378-80.

Traber MG, Stevens JF. Vitamins C and E: Beneficial effects from a mechanistic perspective. Free Radic Biol Med. 51(5). 1000-13 (2011).

Hamid AA, Aiyelaagbe OO, Usman LA, Ameen OM, Lawal A. Antioxidants: its medicinal and pharmacological applications. Afr J Pure Appl Chem. 4(8). 142-51 (2010).

Devbhuti P, Saha A, Sengupta C. Gentamicin induced lipid peroxidation and its control with ascorbic acid, Act Pol Pharm. 66(4). 363-69 (2009).

Ray S, Roy K, Sengupta C. Cisplatin-induced lipid peroxidation and its inhibition with Ascorbic acid. Indian J Pharm Sci. 68(2). 199-204 (2006).

Devbhuti P, Saha A, Sengupta C. Effect of ascorbic acid on lipid peroxidation induced by ceftazidime. J Pharm Sci Technol. 1(1). 51-53 (2011).

Ray S. Evaluation of protective role of ascorbic acid on Flutamide-induced lipid peroxidation. Int J Pharm Tech Res. 4(1). 135-40 (2012).

Aruna P, Shruti B, Archana A, Sameer H, Villasrao J. Tobramycin-induced lipid peroxidation and its control with ascorbic acid. Indo American J Pharm Res. 3(8). 6076-82 (2013).

Roy K., De AU, Sengupta C. Evaluation of glutathione and ascorbic acid as suppressors of drug-induced lipid peroxidation, Ind J Exp Biol. 38(6). 580-86 (2000).

Gaweł S, Wardas M, Niedworok E, Wardas P. Malondialdehyde (MDA) as a lipid peroxidation marker. Wiad lek. 57(9-10). 453-55 (2004).

Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014. 1-31 (2014).

Niedernhofer LJ, Daniels JS, Rouzer CA, Greene RE, Marnett LJ. Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cells. J Biological Chem. 278(33). 31426-33 (2003).

Hogg N, Kalyanaraman B. Nitric oxide and lipid peroxidation. Biochimica Et Biophysica Acta. 1411(2-3). 378-84 (1999).

Hilditch TP. The chemical constituents of natural fats. 3rd ed. London: Champel and Hall Ltd; 1956.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 95(2). 351-58 (1979).

Sun J, Zhang X, Broderick M, Fein H. Measurement of nitric oxide production in biological systems by using griess reaction assay. Sensors. 3(8). 276-84 (2003).

Supratim Ray. Exploring the protective role of water extract of Spirulina platensis on flutamide-induced lipid peroxidation using 4-Hydroxy nonenal and nitric oxide as model markers. Rese J Pharm Techn. 4(12). 1857-60 (2011).

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 82(1). 70-7 (1959).

Ray S. Exploring the protective role of ascorbic acid on Busulfan-induced lipid peroxidation. Res J Pharm, Biol Chem Sci. 2(4). 702-8 (2011).

Remington: The science and practice of pharmacy. In: Joseph PR, Paul B, editors. Statistics. 21st ed. Philadelphia: Lippincott Williams & Wilkins; 2006. p. 127-50.